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Do pregnancies reduce iron overload in HFE hemochromatosis women? results from an observational prospective study
- Virginie Scotet†1Email author,
- Philippe Saliou†1, 2,
- Marianne Uguen1,
- Carine L’Hostis1,
- Marie-Christine Merour3,
- Céline Triponey3,
- Brigitte Chanu3,
- Jean-Baptiste Nousbaum1, 4,
- Gerald Le Gac1, 5 and
- Claude Ferec1, 3, 5
© The Author(s). 2018
Received: 11 August 2016
Accepted: 6 February 2018
Published: 17 February 2018
HFE hemochromatosis is an inborn error of iron metabolism linked to a defect in the regulation of hepcidin synthesis. This autosomal recessive disease typically manifests later in women than men. Although it is commonly stated that pregnancy is, with menses, one of the factors that offsets iron accumulation in women, no epidemiological study has yet supported this hypothesis. The aim of our study was to evaluate the influence of pregnancy on expression of the predominant HFE p.[Cys282Tyr];[Cys282Tyr] genotype.
One hundred and forty p.Cys282Tyr homozygous women enrolled in a phlebotomy program between 2004 and 2011 at a blood centre in western Brittany (France) were included in the study. After checking whether the disease expression was delayed in women than in men in our study, the association between pregnancy and iron overload was assessed using multivariable regression analysis.
Our study confirms that women with HFE hemochromatosis were diagnosed later than men cared for during the same period (52.6 vs. 47.4 y., P < 0.001). Compared to no pregnancy, having at least one pregnancy was not associated with lower iron markers. In contrast, the amount of iron removed by phlebotomies appeared significantly higher in women who had at least one pregnancy (eβ = 1.50, P = 0.047). This relationship disappeared after adjustment for confounding factors (eβ = 1.35, P = 0.088).
Our study shows that pregnancy status has no impact on iron markers level, and is not in favour of pregnancy being a protective factor in progressive iron accumulation. Our results are consistent with recent experimental data suggesting that the difference in disease expression observed between men and women may be explained by other factors such as hormones.
HFE-related hemochromatosis (or type 1 hemochromatosis; OMIM #235200) is an inborn iron metabolism disorder that is particularly common in Caucasian populations [1, 2]. This genetic disease is characterised by an inappropriate high iron absorption from enterocytes and by an excessive iron release from macrophages. It is due to a defective regulation of the synthesis of hepcidin, the key regulator of iron homeostasis [3–5]. As human body is not capable of eliminating the excess of iron, this will gradually affect various organs and may result in serious damages, e.g. cirrhosis, hepatocellular carcinoma or cardiomyopathy .
HFE hemochromatosis is inherited as an autosomal recessive trait and is mainly associated with the HFE p.[Cys282Tyr];[Cys282Tyr] genotype . The penetrance of this genotype is clearly incomplete [8–10], and its expression is influenced by genetic and environmental factors that may increase or reduce the iron burden [8, 11, 12].
This expression of this genetic disorder is delayed in women than in men. Moreover, HFE hemochromatosis women present with a less severe clinical profile, notably a lower prevalence of liver injury [13, 14]. This difference between genders has classically been attributed to the protective effects mediated by iron losses related to menses and pregnancy [15–17].
During pregnancy, maternal iron requirements increase substantially to allow for the physiological expansion of the haemoglobin mass, to promote formation of the foetus and placenta, and to cope with blood losses at delivery . While it is commonly stated in the literature that these pregnancy-associated iron losses are one of the factors that offset the lifelong iron accumulation in women [15–17], this assertion has not been supported by any epidemiological studies in humans.
As recent literature has shown that other factors such as hormones may explain the differences observed according to gender [19, 20], we sought to investigate the association between pregnancy and the phenotypic expression of the predominant HFE p.[Cys282Tyr];[Cys282Tyr] genotype.
Study design and participants
This work evaluated a cohort of 140 consecutive p.Cys282Tyr homozygous hemochromatosis women who started phlebotomies between January 1st, 2004 and December 31st, 2011 at a blood centre in western Brittany (Brest, France) where this disease is particularly common [21, 22].
Included patients presented elevated iron markers (with transferrin saturation (TS) > 45% and serum ferritin (SF) > 200 μg/L) and were referred by general practitioners or gastroenterologists to the blood centre for phlebotomy.
This study relied on data obtained using a clinical questionnaire that was filled out upon admission to the phlebotomy program. As previously described , this questionnaire, which was completed by a referral physician, collected information on socio-demographic characteristics (gender, age at diagnosis, etc.), lifestyle factors (height, weight, alcohol intake, etc.) and biological parameters (including transferrin saturation and serum ferritin).
It also recorded data on reproductive functions, on the presence (and number if any) of pregnancies prior to the beginning of the treatment (excluding miscarriages and abortions occurring in the first trimester), as well as on the menopausal status at admission to the phlebotomy program. Patients were also asked if they were regular blood donors, if they had chronic bleedings (including gastrointestinal bleedings, chronic hematuria, bleedings due to parasitic infections) and if they received blood transfusions (and how many if any), all this prior to admission to the phlebotomy program.
At the end of the depletion stage, treatment-related data (i.e. the number and average volume of the phlebotomies) were recorded. These data enable estimation of the amount of iron removed (AIR; in grams) to normalise patient’s iron stores (i.e. to reach SF < 100 μg/L). This calculation was performed assuming that 1 L of blood contains 0.5 g of iron .
Statistical analysis was carried out using the SAS software (version 9.4; SAS Institute Inc., Cary, NC). All tests were performed two-sided, and the significance level was set at 5% for all analyses.
First, we described the baseline characteristics of the studied population. Continuous variables were described in means and standard deviation (SD), and were compared using Student’s t test or Anova. When these variables were not normally distributed, they were described by median and interquartile range (IQR), and compared by the Mann-Whitney test. Categorical variables were summarised in percentages, and were compared using χ2 test or Fisher’s exact test when appropriate.
Before assessing the influence of pregnancy, we explored the impact of gender on disease expression. For this, we compared the age at diagnosis of women in the study to that observed in the men cared for during the same period in our blood centre (n = 161) (using Student’s t test). We also evaluated whether the proportion of diagnosed female patients increased with the age at diagnosis i.e. if the sex ratio (M/F) decreased with the age at diagnosis (using a linear trend χ2 test).
In a second time, we compared women characteristics according to their pregnancy status (number of previous pregnancies). We then investigated the association between pregnancy and the degree of iron overload (assessed by SF and AIR) using linear regression analysis. As the distributions of these quantitative dependant variables were highly skewed (Kolmogorov-Smirnov test), we performed logarithmic (ln) transformations to normalise them. With such a transformation, the exponential of the estimated regression coefficient (eβ) indicates how many times the outcome variable varies for each unit increment in the explanatory variable. This means, in other words, that each unit increment in the explanatory variable multiplies the expected value of Y by eβ. We then tested the association between the iron parameters and potential confounding factors such as age at diagnosis, alcohol intake (whose hepatotoxic effect increases the disease severity) [24, 25]) and menopausal status. We thereafter fitted a multivariable model to enable adjustment for confounders. All explanatory variables associated with the outcome variables at a conservative threshold of P < 0.20 in the univariable analysis were included in this multivariable analysis.
In the present study, excessive alcohol intake was defined, in accordance with the World Health Organization definition, as daily consumption exceeding two glasses per day (i.e. 14 glasses per week) for women. Overweight status was defined as a body mass index (BMI) ≥ 25 kg/m2.
Baseline characteristics of the study population
Baseline characteristics of the p.Cys282Tyr homozygous hemochromatosis women according to the number of pregnancies that women had prior to entry into the phlebotomy program
Number of pregnancies: 0
Number of pregnancies: 1 or 2
Number of pregnancies: ≥ 3
Number of women
Age at diagnosis (n = 137)
≥ 60 ya
< 60 y.
Body mass index (n = 131)
≥ 25 kg/m2
< 25 kg/m2
Alcohol intake (n = 134)
Menopause (n = 126)
Regular blood donations (n = 136)
Chronic bleedings (n = 135)
Blood transfusions (n = 134)
Analysis of the gender difference in expression of the p.[Cys282Tyr];[Cys282Tyr] genotype
Distribution of men and women by age at diagnosis
Age at diagnosis
< 40 y.
≥ 60 y.
Baseline characteristics of women according to the pregnancy status
The number of pregnancies of the 137 p.Cys282Tyr homozygous hemochromatosis women ranged from zero to six, with an average of 2.4 pregnancies per woman (± 1.3). Nearly 90% of the women in our sample (n = 123) had at least one pregnancy before entering the phlebotomy program, with the majority of women having had two (42.3%; n = 58) or three (27.0%; n = 37) pregnancies. Approximately 15% of the women had four pregnancies or more (15.3%; n = 21).
Biological parameters of the p.Cys282Tyr homozygous hemochromatosis women according to the number of pregnancies that women had prior to entry into the phlebotomy program
Number of pregnancies: 0
Number of pregnancies: 1 or 2
Number of pregnancies: ≥ 3
Transferrin saturation (%) (n = 137)
Serum ferritin (μg/L) (n = 137)
Amount of iron removed (g) (n = 127)
Hemoglobin (g/dL) (n = 108)
Association between pregnancy and iron markers
Results of the linear regression analysis modelling the association between pregnancies and serum ferritin
No. of pregnancies
1 or 2
Age at diagnosis
< 60 y.a
≥ 60 y.
Body mass index
< 25 kg/m2
≥ 25 kg/m2
Regular blood donations
Results of the linear regression analysis modelling the association between pregnancies and the amount of iron removed by phlebotomies
No. of pregnancies
1 or 2
Age at diagnosis
< 60 y.a
≥ 60 y.
Body mass index
< 25 kg/m2
≥ 25 kg/m2
Regular blood donations
Similar findings were obtained for the second iron marker: AIR (Table 5). The univariable analysis revealed no significant association between pregnancy and AIR. When comparing women who had at least one pregnancy to women with no pregnancy, AIR was even significantly higher (i.e. 1.5 time higher) in women who had at least one pregnancy (eβ≥1 vs. 0 pregnancy = 1.50; 95% CI: 1.01–2.23, P = 0.047). However, this relationship became non-significant after adjustment for potential confounders (eβ≥1 vs. 0 pregnancy = 1.35; 95% CI: 0.96–1.90, P = 0.088). The results remained still at the limit of significance after adjustment for age at diagnosis, alcohol consumption, menopausal status and baseline SF level, when comparing women having one or two pregnancies to women having no pregnancy (P = 0.058).
Pregnancy has been suggested to be one potential factor responsible for the later manifestation of HFE hemochromatosis in women [15–17]. Yet, our work is the first epidemiological study entirely devoted to the analysis of the association between pregnancy and the phenotypic expression of the main HFE genotype in humans. Our study confirms that p.Cys282Tyr homozygous women are diagnosed at a later age than men, and thus corroborates the existence of a difference in the expression of this genotype between men and women. Nevertheless, these results do not confirm the protective effect typically attributed to pregnancy to explain the slower iron accumulation in women.
Our study was subject to little selection bias for several reasons. First, it was a cohort study that included prospectively almost all of the p.Cys282Tyr homozygous hemochromatosis women enrolled in a phlebotomy program at our centre over the study period. Second, the rate of missing values for the main explanatory variable (pregnancy) was very low (~ 2.0%), making our sample fully representative of the p.Cys282Tyr homozygous women who come to medical attention in our area. Finally, we also ensured that the baseline characteristics of women excluded from the multivariable analysis (due to missing values) did not differ from those of included women.
Moreover, iron burden was measured using SF but also AIR, which is the reference method to assess body iron stores . AIR is a more reliable marker than SF, which may also be increased beyond the real degree of iron burden by secondary causes of hyperferritinaemia as excessive alcohol intake or metabolic or inflammatory syndromes .
Our study was also able to take into account major confounders, as alcohol intake or menopausal status at entry into the phlebotomy program. Nevertheless, we did not have information on the presence of some other factors susceptible to modify the iron burden (iron supplementation during pregnancy, blood losses from labor and delivery, postpartum hemorrhage, pregnancy complications (pre-eclampsia, abruption, placenta previa), importance of menstrual blood losses, ...) . However, most of these data are not easy to quantify precisely. Some of them may also only have a small effect on iron status, as maternal breastfeeding because its duration (with its consecutive amenorrhea) is in average relatively short and because very little iron is transferred to the milk. We are also aware that it would have been ideal to know the delay between various pregnancies, as well as between the last pregnancy and the beginning of the treatment.
To the best of our knowledge, no epidemiological study has so far been exclusively devoted to the study of the relationship between pregnancy and iron overload in HFE hemochromatosis in humans. Some studies have nevertheless shown interest to pregnancy. In a study comparing clinical features of 176 women and 176 matched men , Deugnier et al. mentioned that they found no significant correlation between the number of pregnancies and the hepatic iron concentration or the AIR, but no data were presented. They also observed, through a population-based screening study , that the number of pregnancies did not differ between 23 expressive and 19 non expressive women. More recently, the same team explored pregnancy as a potential confounder in a model assessing the relationship between body mass index and iron burden in HFE hemochromatosis . They found no significant association between the number of pregnancies and AIR (≥ 6 g or < 6 g) in univariable analysis. All these data are consistent with our results.
Our findings seem quite plausible in the current context of fertility. Given the 2016 French fertility rate estimated at 1.93 children per woman , it is not surprising that iron losses resulting from an average of two pregnancies per woman are not sufficient to protect against this disease. This situation was most likely different in the past when the fertility rate was higher.
During pregnancy, the daily requirements for absorbed iron markedly increase, from approximately 0.8 mg/day in the first trimester to ~ 8 mg/day in the third trimester [18, 31–33]. Globally, for a singleton pregnancy, a woman needs up to one gram of iron to ensure the balance of iron (depending on iron stores at the beginning of gestation). This corresponds to ~ 500 mg for the physiological expansion of haemoglobin mass, ~ 315 mg for the constitution of foetal tissue and placenta and ~ 250 mg for basal losses [32, 34, 35]. Blood losses at delivery also account to about 150 to 250 mg iron. These additional needs are drawn from the reserves of the mother and are transported to the foetus via the placenta. To cope with extra needs and to replenish the maternal stores, intestinal iron absorption also increases during pregnancy (about approximately 25%) . A part of iron is also made available from the stopping of menses (although this is not sufficient) and from prophylactic iron supplementation that is usually recommended.
It would have been interesting to compare the amount of iron lost during pregnancy to that lost during menses. For example, it has been shown that healthy women with normal menses lose an average of 26 to 65 mL of blood per cycle [37–39], which corresponds at most to a loss of 1 mg iron per cycle (according to the recent assays performed by Napolitano et al.) (20) . Therefore, if we consider that the entire childbearing period lasts average of 40 years (from the mean age at menarche (~ 12 y.) until the mean age at menopause (51 y.)), the total quantity of iron lost due to menses over a lifetime is approximately 520 mg (assuming 13 cycles of 28 days per year). This quantity appears lower than that lost during one pregnancy.
Our findings are also consistent with the results of an experimental study in a mouse model  showing that multiple pregnancies do not reduce body iron stores in Hfe−/− mice. This study found that all relevant clinical parameters of hemochromatosis (except TS) were not significantly decreased (or even increased) in multiparous females compared with nulliparous females. The hepatic expression of hepcidin  and its regulator (BMP6)  was reduced in multiparous females, suggesting that the inhibition of intestinal iron absorption was inactivated in response to pregnancy.
Current experimental data suggest that other factors such as hormonal factors may explain the difference in disease expression observed between men and women. Recent findings revealed that the gender difference observed in diseases associated with altered hepcidin expression such as HFE hemochromatosis may be explained by the negative regulation of hepcidin transcription by testosterone . Latour et al. showed that testosterone inhibits hepcidin transcription in mice, via enhancement of epidermal growth factor (EGF) receptors signalling in the liver (knowing that EGF was recently shown to inhibit liver hepcidin synthesis ). The authors stated that the selective inhibition of EGF receptor in male mice stops testosterone-induced repression and clearly increases hepcidin expression. Moreover, castration of male mice enhances hepcidin expression thus lowers iron overload. Therefore this work highlights that testosterone should be one major hormone responsible for the observed gender difference in regulation of iron metabolism.
Our work challenges an old and well-established, yet unproven, hypothesis that pregnancy slows iron accumulation in women with HFE hemochromatosis. Combined with recent experimental data from the literature, our findings clearly show that the effect of pregnancy is not as important as initially announced and that the search for the factors responsible for the gender difference should continue.
The authors are grateful to Pascal Trouvé (Inserm UMR 1078, Brest, France) and to Zarrin Alavi (EA3878, Inserm, CIC 1412, Brest, France) for reviewing the manuscript.
This study was supported by a grant from the Etablissement Français du Sang. The funding had no role in the study design, in the collection, analysis, and interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication.
Availability of data and materials
The data that support the findings of this study are available from Pr. Claude Ferec but restrictions apply to the availability of these data, which were used under license for the current study, and so are not publicly available. Data are however available from the authors upon reasonable request and with permission of Pr. Claude Ferec.
VS and CF conceived and designed the study. VS wrote the first draft of the manuscript. VS, PS and MU analysed and interpreted the data. CLH performed the additional analyses requested by the reviewers and interpreted the data. CT and BC were responsible for patients’ inclusion and data acquisition. MCM managed the data. GLG helped in interpreting findings and in drafting the manuscript. JBN helped in interpreting findings and critically revised the manuscript for important intellectual content. All authors revised the manuscript and approved the final manuscript.
Ethics approval and consent to participate
This study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethical Committee of the University Hospital of Brest (n# 2003_15-12_EMI 0115). Written informed consent was obtained from each participant.
Consent for publication
The authors declare that they have no competing interests.
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