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Assisted reproductive technology and the risk of preeclampsia: an updated systematic review and meta-analysis



The objective of this systematic review and meta-analyses was to assess the risk of preeclampsia among women who conceived with assisted reproductive technology (ART).


We searched the ISI Web of Knowledge, Medline/PubMed, Scopus, and Embase (from inception to May 2017) for English language articles using a list of key words. In addition, reference lists from identified studies and relevant review articles were also searched. Data extraction was performed by two authors, and the study quality was assessed using the Newcastle–Ottawa Scale. Random-effects model meta-analysis was applied to pool the relative risks (RR) across studies.


A total of 48 studies (5 case-control studies and 43 cohort studies) were included in this meta-analysis. The Cochran Q test and I2 statistics revealed substantial heterogeneity (Q = 26,313.92, d.f. = 47, p < 0.001 and I2 = 99.8%). Meta-analysis showed a significant increase in preeclampsia in women who conceived by ART compared with those who conceived spontaneously (RR = 1.71, 95% CI = 1.11–2.62, p = 0.015).


The findings of this systematic review indicate that the use of ART treatment is associated with a 1.71-fold increase in preeclampsia.

Peer Review reports


Assisted reproductive technologies (ART) are used to treat infertility problems and contain methods in which oocyte and sperm are manipulated in vitro [1]. The use of ART has increased exponentially worldwide and is responsible for over than one million births annually [2, 3]. Having been treated by ART, the women who conceived had numerous adverse outcomes, both for themselves and the infants [3]. Previous studies have demonstrated that ART is associated with small for gestational age infants, preterm delivery, perinatal mortality, preeclampsia (PE), gestational diabetes, placenta previa, placental abruption, and cesarean delivery [4]. Of several adverse pregnancy consequences, hypertensive disorders affect 6–8% of all pregnancies through gestational hypertension and PE [5, 6]. In contrast to spontaneous pregnancy, pregnancies with ART are at an increased risk of PE [7]. It remained unclear whether either ART itself [in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), intrauterine insemination (IUI), oocyte donation (OD), or embryo donation (ED)] or maternal risk factors associated with ART (that is, advanced maternal age, obesity, change of partner, longer interval between births, reduced smoking, and chronic hypertension) were related to increased risk of PE [7, 8]. Some studies have shown the probability of the taking of some medications during pregnancy, such as low-dose aspirin, [9] prevents for PE in high-risk women [10,11,12]. Thus, identifying high-risk women during the early period of gestation will be worthwhile for the prevention and management of the pregnancy complications [13]. Finally, the lack of diagnostic criteria for pregnancy complications associated with hypertension, especially for PE, make the research in this field more complicated [14].

In the present paper, the authors conducted a comprehensive systematic review of ART procedures and PE. The aim of this review was to investigate whether ART mediated pregnancies (i.e., IUI, IVF, ICSI, OD, and ED) increased the incidence of PE in pregnancy compared with spontaneous pregnancies.


Search strategy

This meta-analysis was performed according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) checklist [15]. We conducted a systematic literature search in Medline/PubMed, Embase, Scopus, and the ISI Web of Knowledge from inception through June 2017 for studies examining the association between ART and PE. In addition, reference lists from all retrieved papers were checked. Table 1 provides more details about the search strategy.

Table 1 Search strategy for MEDLINE (MeSH, Medical Subject Headings)

Inclusion and exclusion criteria

We included published case-control studies and cohort studies evaluating the association between ART and PE risk. No geographic restrictions were used. The following types of studies were excluded: (a) non-English full-text studies, (b) animal studies, (c) repeated or overlapping studies, (d) reviews, meta-analysis and cross-sectional articles, case reports, editorials, and letters to the editor, (e) abstract-only publications or unpublished studies. There were five case-control studies added to the study. However, it was not substantially possible to estimate the relative risk (RR) with case-control design due to the fact that the marginal probabilities were not available; under the rare disease assumption, the odds ratio will be approximate the RR.

Outcome and exposure

In the present study, all types of ART treatments were considered as the interested exposure variable. Our outcome was PE defined as “elevated blood pressure (BP) (more than 140/100 mmHg) and proteinuria (0.3 g over 24 hours or more).”

Data extraction

Two authors (MM and SM) independently extracted the following data from all studies meeting the inclusion criteria: first author’s name, year of publication, location, study period, design, sample size, and study results. In addition, outcome data were extracted from each study in a 2 × 2 table, and the results were expressed as RR with their respective 95% confidence intervals (CIs) [9].

Quality assessment

Two authors (MM and SM) independently assessed the quality of studies using the Newcastle–Ottawa Scale (NOS) [16]. This scale assesses methodology in three domains: (a) selection of study groups, (b) comparability of groups, and (c) ascertainment of exposure and outcomes. Total score ranged from 0 to 9 with a score of ≥8 indicating high quality.

Statistical analysis

Statistical analysis was conducted using Stata version 13.0 (Stata Corp, College Station, TX, USA). The RR was used as the effect size of association across studies. The Cochran Q test and the I2 statistic were used to evaluate heterogeneity among studies [17]. Concerning the Cochrane Q test, P < 0.10 was deemed statistically significant for heterogeneity. The I2 statistic indicates the percentage of total variation across studies that is due to heterogeneity rather than chance and is classified as mild (25%), moderate (50%), or high (75%) [17]. The Galbraith plot was used to detect the potential sources of heterogeneity [18]. The pooled RR estimate and corresponding 95% CI were calculated by using the random-effect model incorporating between-study variability. The Begg’s rank correlation test, Egger’s weighted regression test, and visual inspection of a funnel plot were used to assess publication bias [19, 20]. All tests were two-tailed and a P value of < 0.05 was deemed statistically significant.


Study selection

The process of study selection is illustrated in Fig. 1. A total of 1244 relevant papers were identified using diverse search strategies in four databases (113 from PubMed, 140 from Embase, 897 from Scopus, and 94 from Web of Knowledge) and three records of gray literature. After removing duplicates, 1057 papers remained, and 749 papers were deemed ineligible after title and abstract screening, and 308 relevant papers were considered for further screening through full-text reading. After the exclusion of all non-eligible studies (n = 260), a total of 48 studies (5 case-control studies and 43 cohort studies) were included in this meta-analysis.

Fig. 1
figure 1

Flow diagram of study process

Study characteristics

For each study, sample size, total number of ART and non-ART group, number of PE cases in each group, publication date, first author, target country, type of study, and participant mean age of each group were extracted. Cross-sectional studies and non-English studies were excluded from the meta-analysis. All of the primary studies were published between 1999 and 2017 and out of 48 studies, 11 were carried out in the United States, 11 in Asia, and 26 in Europe. The characteristics of studies considered in the meta-analysis are presented in Table 2.

Table 2 Characteristics of the primary studies included in the meta-analysis

Quantitative data synthesis

A total of 156,246 ART cases (with 14,560 cases of PE) and 6,558,249 non-ART cases (with 202,064 cases of PE) were included in the analysis. Risk ratios and their 95% CIs were reported using the Mantel–Haenszel method. The relationship of ART and the risk of PE were estimated using the 48 primary included studies. The pooled estimate of RR in this meta-analysis revealed that ART was significantly associated with a higher risk of PE (pooled RR = 1.708, 95% CI = 1.111–2.624, z = 2.44, p = 0.015), that is, the PE risk in ART group was 1.687 times greater compared to the non-ART group (Fig. 2, Table 3).

Fig. 2
figure 2

Forest plot showing effect of ART on preeclampsia

Table 3 Summary of meta-analysis results and subgroups analysis

Heterogeneity analysis

Chi-square analysis showed that there was substantial heterogeneity between primary studies (heterogeneity χ2 = 26,313.92, p < 0.001, I2 = 99.8%, and τ2 = 2.17). Therefore, we concluded that the random-effect model was used to pool the studies. To discover the source of heterogeneity, subgroup analysis was carried out on the basis of study design (case control and cohort), study region (United States, Asia, and Europe), and study period (1999–2010 and 2010–2017) (Figs. 3, 4 and 5, and Table 3). After subgroup analysis, heterogeneity across studies did not decrease effectively; therefore, all estimations of RR were made by the random-effect model.

Fig. 3
figure 3

Forest plot showing effect of ART on preeclampsia based on study design

Fig. 4
figure 4

Forest plot showing effect of ART on preeclampsia based on study period

Fig. 5
figure 5

Forest plot showing effect of ART on preeclampsia based on regions

Risk of publication bias

Both graphical and statistical assessments were performed to check for the presence of publication bias. On the basis of the asymmetrical funnel plot (Fig. 6) and Begg’s test (p = 0.001), there was evidence of publication bias in this study. Accordingly, we excluded non-English papers from the meta-analysis and this can lead to bias.

Fig. 6
figure 6

Funnel plots of studies examining the association between ART and preeclampsia


This study aimed to evaluate whether several studies agree with the effect of ART on the presence of PE. In this meta-analysis, 6,714,495 cases were recruited (156,246 ART cases and 6,558,249 non-ART cases). To detect the risk of PE regarding the use of ART, the heterogeneity among the studies was assessed, and the appropriate statistical tool was applied. To increase the validity of the results, the risk of publication bias was checked. Analysis of the important subgroups, such as publication date, type of study, and region, was performed.

Similar to the results achieved from our study, most of the studies have introduced the use of ART as a significant risk factor for placental abruption, low and very low birth weight in infants, placenta previa, gestational hypertension, risk of cesarean section, and PE [21, 22]. However, not all the investigators agree with the adverse effect of ART on pregnancy outcomes [23, 24]. Most of previous studies have proven the important impact of using ART on PE [25,26,27,28]. The positive association between ART and PE is well demonstrated by the included studies. Regarding the magnitude of the effect size, the pooled results from case-control studies were in compliance with those of cohort studies. However, in contrast to the cohort studies, the pooled RR from the case-control studies was not statistically significant. Moreover, the impact of ART on PE did not differ in two distinct periods of time (2010 as the cut-off point). Although consistent results were observed among different regions, the pooled RR from the European studies was not significant. Moreover, the effect size of the Asian and United States studies was higher than that of Europe.

We found that the use of ART was a significant risk factor for PE. The application of ART has increased across many countries around the world as a way to cope with infertility problems. The prevalence of using ART differs among countries. Annually, more than 1.5% of all births in the United States are the result of ART. The prevalence of PE is almost 10% in Africa and 15% in China [29,30,31,32]. In addition, the prevalence of PE has an increasing slope. Numerous factors, including the use of ART, hypertension, diabetes, obesity, and early diagnosis problems, are responsible for the ascending trend of PE prevalence [30, 33]. The adverse outcomes after ART cause damage to body organs, such as the kidney and liver, through PE as well as maternal mortality, perinatal deaths, preterm birth, intrauterine growth restriction, bleeding problems, and fetal growth retardation [34, 35]. In addition to ART, other factors such as anti-phospholipid syndrome, previous PE, family history of PE, insulin-dependent diabetes, obesity, multiple pregnancies, and nulliparity can affect PE [36]. The mechanism in which ART affects PE is not well known. However, it has been argued that abnormal placentation can influence PE. In some ART procedures, the blood flow is compromised and is diminished, which is then followed by obstetric complications. Moreover, placental insufficiency is caused by the transfer of the conceptus into the uterine cavity and the impact of the altered hormonal environment in the endometrium where the development of the maternal–fetal interface can be influenced [37, 38]. It has been argued that ART may have epigenetic effects. The pregnancies from ART are associated with PE through oxidative stress. In addition, ART has several types of reproductive dysfunction with the same strength as miscarriages. Recurrent spontaneous miscarriages, along with infertility treatments, increase the risk of PE in comparison to those without treatment [39]. Nonetheless, the excess RR in the association between ART and PE can be caused by multiple factors, such as previous fertility complications, lifestyle, smoking habits, long inter-birth intervals, multiple pregnancy, and advanced maternal age [39]. However, there are many other causal factors associated with infertility itself in which the relationship between PE and ART can be argued.

Thomopoulos et al. assessed the risk of hypertensive disorders in pregnancy following ART using an overview of the studies conducted from 1978 to 2016 [40]. Their study included papers from PubMed and the Cochrane Collaboration Library databases with a total of 32 papers with PE as an outcome. The present meta-analysis has added primary studies from other databases such as Embase, Scopus, and ISI Web of Knowledge with a total number of 48 papers up to June 2017.

The controversy of using statistical tools to determine the magnitude of heterogeneity in meta-analysis has several potential causes, including sample size and number of the included studies, the period of time, the geographical patterns, the level of development, and the types of studies, etc. In this regard, a non-significant result from a chi-square test must not be taken as evidence of a lack of heterogeneity. Furthermore, the chi-square test is very powerful when many studies are included in a meta-analysis. The other statistical tool to detect heterogeneity, the I2 value, depends on the magnitude of the rates [41]. In our meta-analysis, the result of the chi-square test was confirmed by the I2 test. Except for a region of Asia, significant heterogeneities were observed among the pooled and subgroup RRs. The source of heterogeneities may be due to the diversity in the ethnic and cultural conditions and uneven development regions.

However, this study has some limitations. Almost every meta-analysis study deals with uncontrolled confounders. Researchers are not able to control the analysis for the confounders unless the proper information is presented by the original articles. To overcome this problem, “individual patient or participant data (IPD)” is suggested in which requires the detailed information and data-sets from every single original article and it is not applicable in most of the cases regarding that the authors (original articles) might not be interested to present their data and other potential reasons.

This systematic review has several limitations. First, the most important limitation for this study as for other systematic review is the lack of data for subgroup analysis based on type of pregnancy (singleton versus twin pregnancy) or for data analysis controlling for known confounders. Second, our study included only English full-text papers. However, globally published papers might present higher quality research compared with those of local origin.


The present systematic review and meta-analysis revealed that the use of ART increases the risk of PE considerably. More attention must be paid to Asia and the United States, where the association is stronger and significant.



Assisted Reproductive Technology


Confidence Interval




Relative Risk


  1. Zegers-Hochschild F, Adamson GD, Dyer S, Racowsky C, de Mouzon J, Sokol R, Rienzi L, Sunde A, Schmidt L, Cooke ID, et al. The International Glossary on Infertility and Fertility Care, 2017. Fertil Steril. 2017;108(3):393–406.

  2. Davies M, Rumbold A, Moore V. Assisted reproductive technologies: a hierarchy of risks for conception, pregnancy outcomes and treatment decisions. J Dev Orig Health Dis. 2017;8(4):443–7.

    Article  CAS  PubMed  Google Scholar 

  3. Stephen EH, Chandra A, King RB. Supply of and demand for assisted reproductive technologies in the United States: clinic-and population-based data, 1995–2010. Fertil Steril. 2016;105(2):451–8.

    Article  PubMed  Google Scholar 

  4. Stern JE, Luke B, Tobias M, Gopal D, Hornstein MD, Diop H. Adverse pregnancy and birth outcomes associated with underlying diagnosis with and without assisted reproductive technology treatment. Fertil Steril. 2015;103(6):1438–45.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Program NHBPE. Report of the national high blood pressure education program working group on high blood pressure in pregnancy. Am J Obstet Gynecol. 2000;183(1):s1–s22.

    Article  Google Scholar 

  6. Paré E, Parry S, McElrath TF, Pucci D, Newton A, Lim K-H. Clinical risk factors for preeclampsia in the 21st century. Obstet Gynecol. 2014;124(4):763–70.

    Article  PubMed  CAS  Google Scholar 

  7. Sibai B. Subfertility/infertility and assisted reproductive conception are independent risk factors for pre-eclampsia. BJOG. 2015;122(7):923.

    Article  CAS  PubMed  Google Scholar 

  8. Sibai B, Dekker G, Kupferminc M. Pre-eclampsia. Lancet. 2005;365(9461):785–99.

    Article  PubMed  Google Scholar 

  9. Murray CJL, Vos T, Lozano R, Naghavi M, Flaxman AD, Michaud C, Ezzati M, Shibuya K, Salomon JA, Abdalla S, et al. Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990-2010: a systematic analysis for the global burden of disease study 2010. Lancet. 2012;380(9859):2197–223.

    Article  PubMed  Google Scholar 

  10. Askie LM, Duley L, Henderson-Smart DJ, Stewart LA. Antiplatelet agents for prevention of pre-eclampsia: a meta-analysis of individual patient data. Lancet. 2007;369(9575):1791–8.

    Article  CAS  PubMed  Google Scholar 

  11. Villa P, Kajantie E, Räikkönen K, Pesonen AK, Hämäläinen E, Vainio M, Taipale P, Laivuori H. Aspirin in the prevention of pre-eclampsia in high-risk women: a randomised placebo-controlled PREDO trial and a meta-analysis of randomised trials. BJOG. 2013;120(1):64–74.

    Article  CAS  PubMed  Google Scholar 

  12. Women's NCCf, Health Cs: Hypertension in pregnancy: the management of hypertensive disorders during pregnancy. 2010.

    Google Scholar 

  13. Muto H, Yamamoto R, Ishii K, Kakubari R, Takaoka S, Mabuchi A, Mitsuda N. Risk assessment of hypertensive disorders in pregnancy with maternal characteristics in early gestation: a single-center cohort study. Taiwan J Obstet Gynecol. 2016;55(3):341–5.

    Article  PubMed  Google Scholar 

  14. Tranquilli A, Dekker G, Magee L, Roberts J, Sibai B, Steyn W, Zeeman G, Brown M. The classification, diagnosis and management of the hypertensive disorders of pregnancy: a revised statement from the ISSHP. Pregnancy Hypertens. 2014;4(2):97–104.

    Article  CAS  PubMed  Google Scholar 

  15. Moher D, Liberati A, Tetzlaff J, Altman DG. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. Ann Intern Med. 2009;151(4):264–9.

    Article  PubMed  Google Scholar 

  16. Wells G, Shea B, O’Connell D, Peterson J, Welch V, Losos M. The Newcastle–Ottawa Scale (NOS) for assessing the quality if nonrandomized studies in meta-analyses. 2010.

  17. Higgins J, Thompson SG. Quantifying heterogeneity in a meta-analysis. Stat Med. 2002;21(11):1539–58.

    Article  PubMed  Google Scholar 

  18. Galbraith R. A note on graphical presentation of estimated odds ratios from several clinical trials. Stat Med. 1988;7(8):889–94.

    Article  CAS  PubMed  Google Scholar 

  19. Begg CB, Mazumdar M. Operating characteristics of a rank correlation test for publication bias. Biometrics. 1994:1088–101.

  20. Egger M, Smith GD, Schneider M, Minder C. Bias in meta-analysis detected by a simple, graphical test. BMJ. 1997;315(7109):629–34.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Shevell T, Malone FD, Vidaver J, Porter TF, Luthy DA, Comstock CH, Hankins GD, Eddleman K, Dolan S, Dugoff L. Assisted reproductive technology and pregnancy outcome. Obstet Gynecol. 2005;106(5, Part 1):1039–45.

    Article  PubMed  Google Scholar 

  22. Stephansson O, Kieler H, Granath F, Falconer H. Endometriosis, assisted reproduction technology, and risk of adverse pregnancy outcome. Hum Reprod. 2009;24(9):2341–7.

    Article  PubMed  Google Scholar 

  23. Arıoğlu Aydın Ç, Aydın S, Serdaroğlu H. Multifetal gestations with assisted reproductive technique before the single-embryo transfer legislation: obstetric, neonatal outcomes and congenital anomalies. J Matern Fetal Neonatal Med. 2016;29(15):2474–9.

    Google Scholar 

  24. Apantaku O, Chandrasekaran I, Bentick B. Obstetric outcome of singleton pregnancies achieved with in vitro fertilisation and intracytoplasmic sperm injection: experience from a district general hospital. J Obstet Gynaecol. 2008;28(4):398–402.

    Article  CAS  PubMed  Google Scholar 

  25. Guilbaud L, Santulli P, Studer E, Gayet V, Goffinet F, Le Ray C: Impact of oocyte donation on perinatal outcome in twin pregnancies. Fertil Steril. 2017;107(4):948–53.

  26. Valenzuela-Alcaraz B, Crispi F, Manau D, Cruz-Lemini M, Borras A, Balasch J, Gratacos E. J Matern fetal neonatal med. J Matern Fetal Neonatal Med. 2016;29(23):3879–84.

    Article  PubMed  Google Scholar 

  27. Johnston R, Fong A, Lovell S, Sobolewski PS, Rad S, Turner A. Demographic and obstetric outcomes of pregnancies conceived by assisted reproductive technology (ART) compared to non-ART pregnancies. J Bras Reprod Assist. 2015;19(1):16–20.

    Google Scholar 

  28. Lehnen H, Schafer S, Reineke T, Puchooa A, Maiwald R, Zechner U. Twin pregnancies conceived spontaneously and by ART (assisted reproductive technologies) - a retrospective analysis and review. Geburtshilfe Frauenheilkd. 2011;71(8):669–76.

    Article  Google Scholar 

  29. Nakimuli A, Chazara O, Byamugisha J, Elliott AM, Kaleebu P, Mirembe F, Moffett A. Pregnancy, parturition and preeclampsia in women of African ancestry. Am J Obstet Gynecol. 2014;210(6):510–520. e511.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Ananth CV, Keyes KM, Wapner RJ. Pre-eclampsia rates in the United States, 1980-2010: age-period-cohort analysis. BMJ. 2013;347:f6564.

    Article  PubMed  PubMed Central  Google Scholar 

  31. Ye C, Ruan Y, Zou L, Li G, Li C, Chen Y, Jia C, Megson IL, Wei J, Zhang W. The 2011 survey on hypertensive disorders of pregnancy (HDP) in China: prevalence, risk factors, complications, pregnancy and perinatal outcomes. PLoS One. 2014;9(6):e100180.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  32. Sunderam S, Chang J, Flowers L, Kulkarni A, Sentelle G, Jeng G, Macaluso M. Assisted reproductive technology surveillance—United States, 2006. MMWR Surveill Summ. 2009;58(5):1–25.

    PubMed  Google Scholar 

  33. Jeyabalan A. Epidemiology of preeclampsia: impact of obesity. Nutr Rev. 2013;71(suppl 1):S18–25.

    Article  PubMed  Google Scholar 

  34. Noris M, Perico N, Remuzzi G. Mechanisms of disease: pre-eclampsia. Nat Rev Nephrol. 2005;1(2):98–114.

    Article  CAS  Google Scholar 

  35. Redman CW, Sargent IL. Latest advances in understanding preeclampsia. Science. 2005;308(5728):1592–4.

    Article  CAS  PubMed  Google Scholar 

  36. English FA, Kenny LC, McCarthy FP. Risk factors and effective management of preeclampsia. Integr Blood Press Control. 2015;8:7.

    PubMed  PubMed Central  Google Scholar 

  37. Daniel Y, Schreiber L, Geva E, Amit A, Pausner D, Kupferminc M, Lessing J. Do placentae of term singleton pregnancies obtained by assisted reproductive technologies differ from those of spontaneously conceived pregnancies? Hum Reprod. 1999;14(4):1107–10.

    Article  CAS  PubMed  Google Scholar 

  38. Thomopoulos C, Tsioufis C, Michalopoulou H, Makris T, Papademetriou V, Stefanadis C. Assisted reproductive technology and pregnancy-related hypertensive complications: a systematic review. J Hum Hypertens. 2013;27(3):148.

    Article  CAS  PubMed  Google Scholar 

  39. Tandberg A, Klungsøyr K, Romundstad L, Skjaerven R. Pre-eclampsia and assisted reproductive technologies: consequences of advanced maternal age, interbirth intervals, new partner and smoking habits. BJOG. 2015;122(7):915–22.

    Article  CAS  PubMed  Google Scholar 

  40. Thomopoulos C, Salamalekis G, Kintis K, Andrianopoulou I, Michalopoulou H, Skalis G, Archontakis S, Argyri O, Tsioufis C, Makris TK, et al. Risk of hypertensive disorders in pregnancy following assisted reproductive technology: overview and meta-analysis. J Clin Hypertens. 2017;19(2):173–83.

    Article  Google Scholar 

  41. Higgins JP, Green S. Cochrane handbook for systematic reviews of interventions. Hoboken: Wiley; 2011.

  42. Hoy J, Venn A, Halliday J, Kovacs G, Waalwyk K. Perinatal and obstetric outcomes of donor insemination using cryopreserved semen in Victoria, Australia. Hum Reprod. 1999;14(7):1760–4.

    Article  CAS  PubMed  Google Scholar 

  43. Salha O, Sharma V, Dada T, Nugent D, Rutherford A, Tomlinson A, Philips S, Allgar V, Walker J. The influence of donated gametes on the incidence of hypertensive disorders of pregnancy. Hum Reprod. 1999;14(9):2268–73.

    Article  CAS  PubMed  Google Scholar 

  44. Geipel A, Ludwig M, Germer U, Katalinic A, Diedrich K, Gembruch U. Uterine artery Doppler velocimetry and the outcome of pregnancies resulting from ICSI. Hum Reprod. 2001;16(7):1397–402.

    Article  CAS  PubMed  Google Scholar 

  45. Lynch A, McDuffie R, Murphy J, Faber K, Orleans M. Preeclampsia in multiple gestation: the role of assisted reproductive technologies. Obstet Gynecol. 2002;99(3):445–51.

    PubMed  Google Scholar 

  46. Zaib-un-Nisa S, Ghazal-Aswad S, Badrinath P. Outcome of twin pregnancies after assisted reproductive techniques—a comparative study. Eur J Obstet Gynecol Reprod Biol. 2003;109(1):51–4.

    Article  PubMed  Google Scholar 

  47. Pinborg A, Loft A, Schmidt L, Langhoff-Roos J, Andersen AN. Maternal risks and perinatal outcome in a Danish national cohort of 1005 twin pregnancies: the role of in vitro fertilization. Acta Obstet Gynecol Scand. 2004;83(1):75–84.

    Article  PubMed  Google Scholar 

  48. Luke B, Brown MB, Nugent C, Gonzalez-Quintero VH, Witter FR, Newman RB. Risk factors for adverse outcomes in spontaneous versus assisted conception twin pregnancies. Fertil Steril. 2004;81(2):315–9.

    Article  PubMed  Google Scholar 

  49. Källén B, Finnström O, Nygren K, Otterblad Olausson P, Wennerholm UB. In vitro fertilisation in Sweden: obstetric characteristics, maternal morbidity and mortality. BJOG. 2005;112(11):1529–35.

    Article  PubMed  Google Scholar 

  50. Thomson F, Shanbhag S, Templeton A, Bhattacharya S. Obstetric outcome in women with subfertility. BJOG. 2005;112(5):632–7.

    Article  PubMed  Google Scholar 

  51. Hernández-Díaz S, Werler MM, Mitchell AA. Gestational hypertension in pregnancies supported by infertility treatments: role of infertility, treatments, and multiple gestations. Fertil Steril. 2007;88(2):438–45.

    Article  PubMed  PubMed Central  Google Scholar 

  52. Erez O, Vardi IS, Hallak M, Hershkovitz R, Dukler D, Mazor M. Preeclampsia in twin gestations: association with IVF treatments, parity and maternal age. J Matern Fetal Neonatal Med. 2006;19(3):141–6.

    Article  PubMed  Google Scholar 

  53. Prefumo F, Fratelli N, Soares S, Thilaganathan B. Uterine artery Doppler velocimetry at 11–14 weeks in singleton pregnancies conceived by assisted reproductive technology. Ultrasound Obstet Gynecol. 2007;29(2):141–5.

    Article  CAS  PubMed  Google Scholar 

  54. Chen XK, Wen SW, Bottomley J, Smith GN, Leader A, Walker MC. In vitro fertilization is associated with an increased risk for preeclampsia. Hypertension in pregnancy. 2009;28(1):1–12.

    Article  PubMed  Google Scholar 

  55. Sun LM, Walker MC, Cao HL, Yang QY, Duan T, Kingdom JCP. Assisted reproductive technology and placenta-mediated adverse pregnancy outcomes. Obstet Gynecol. 2009;114(4):818–24.

    Article  PubMed  Google Scholar 

  56. Morcel K, Lavoué V, Beuchée A, Le Lannou D, Poulain P, Pladys P. Perinatal morbidity and mortality in twin pregnancies with dichorionic placentas following assisted reproductive techniques or ovarian induction alone: a comparative study. Eur J Obstet Gynecol Reprod Biol. 2010;153(2):138–42.

    Article  PubMed  Google Scholar 

  57. Miyake H, Iwasaki N, Nakai A, Suzuki S, Takeshita T. The influence of assisted reproductive technology on women with pregnancy-induced hypertension: a retrospective study at a Japanese Regional Perinatal Center. J Nippon Med Sch. 2010;77(6):312–7.

    Article  PubMed  Google Scholar 

  58. Suzuki S, Miyake H. Perinatal outcomes of elderly primiparous dichorionic twin pregnancies conceived by in vitro fertilization compared with those conceived spontaneously. Arch Gynecol Obstet. 2010;281(1):87.

    Article  PubMed  Google Scholar 

  59. Yang H, Choi YS, Nam KH, Kwon JY, Park YW, Kim YH. Obstetric and perinatal outcomes of dichorionic twin pregnancies according to methods of conception: spontaneous versus in-vitro fertilization. Twin Res Hum Genet. 2011;14(1):98–103.

    Article  PubMed  Google Scholar 

  60. Kuivasaari-Pirinen P, Raatikainen K, Hippeläinen M, Heinonen S. Adverse outcomes of IVF/ICSI pregnancies vary depending on aetiology of infertility. ISRN Obstet Gynecol. 2012.

  61. Bamberg C, Fotopoulou C, Neissner P, Slowinski T, Dudenhausen JW, Proquitte H, Bührer C, Henrich W. Maternal characteristics and twin gestation outcomes over 10 years: impact of conception methods. Fertil Steril. 2012;98(1):95–101.

    Article  PubMed  Google Scholar 

  62. Lučovnik M, Tul N, Verdenik I, Novak Z, Blickstein I. Risk factors for preeclampsia in twin pregnancies: a population-based matched case-control study. J Perinat Med. 2012;40(4):379–82.

    Article  PubMed  Google Scholar 

  63. Sazonova A, Kallen K, Thurin-Kjellberg A, Wennerholm UB, Bergh C. Obstetric outcome in singletons after in vitro fertilization with cryopreserved/thawed embryos. Hum Reprod. 2012;27(5):1343–50.

    Article  PubMed  Google Scholar 

  64. Mohammed ABF, Abdel-Maaboud M. Obstetric and neonatal outcomes of IVF versus spontaneously conceived dichorionic twins. Middle East Fertil Soc J. 2012;17(4):231–5.

    Article  Google Scholar 

  65. Le Ray C, Scherier S, Anselem O, Marszalek A, Tsatsaris V, Cabrol D, Goffinet F. Association between oocyte donation and maternal and perinatal outcomes in women aged 43 years or older. Hum Reprod. 2012;27(3):896–901.

    Article  PubMed  Google Scholar 

  66. Werder E, Mendola P, Männistö T, O'Loughlin J, Laughon SK. Effect of maternal chronic disease on obstetric complications in twin pregnancies in a United States cohort. Fertil Steril. 2013;100(1):142–149. e142.

    Article  PubMed  PubMed Central  Google Scholar 

  67. Malchau SS, Loft A, Larsen EC, Henningsen A-KA, Rasmussen S, Andersen AN, Pinborg A. Perinatal outcomes in 375 children born after oocyte donation: a Danish national cohort study. Fertil Steril. 2013;99(6):1637–1643. e1633.

    Article  PubMed  Google Scholar 

  68. Revello R, De la Calle M, Moreno E, Duyos I, Salas P, Zapardiel I. J Matern Fetal Neonatal Med. 2013;26(2):193–6.

    Article  PubMed  Google Scholar 

  69. Räisänen S, Randell K, Nielsen HS, Gissler M, Kramer MR, Klemetti R, Heinonen S. Socioeconomic status affects the prevalence, but not the perinatal outcomes, of in vitro fertilization pregnancies. Hum Reprod. 2013;28(11):3118–25.

    Article  PubMed  Google Scholar 

  70. Fong A, Lovell S, Rad S, Turner A, Pan D, Ogunyemi D. 433: obstetrical complications of deliveries conceived by assisted reproductive technology in California. Am J Obstet Gynecol. 2014;210(1):S219–20.

    Article  Google Scholar 

  71. Fox NS, Roman AS, Saltzman DH, Hourizadeh T, Hastings J, Rebarber A. Risk factors for preeclampsia in twin pregnancies. Am J Perinatol. 2014;31(02):163–6.

    PubMed  Google Scholar 

  72. Silberstein T, Levy A, Harlev A, Saphier O, Sheiner E. Perinatal outcome of pregnancies following in vitro fertilization and ovulation induction. J Matern Fetal Neonatal Med. 2014;27(13):1316–9.

    Article  PubMed  Google Scholar 

  73. Morency A-M, Shah PS, Seaward PGR, Whittle W, Murphy KE. Obstetrical and neonatal outcomes of triplet births–spontaneous versus assisted reproductive technology conception. J Matern Fetal Neonatal Med. 2016;29(6):938–43.

    Article  PubMed  Google Scholar 

  74. Lee MS, Cantonwine D, Little SE, McElrath TF, Parry SI, Lim K-H, Wilkins-Haug LE. Angiogenic markers in pregnancies conceived through in vitro fertilization. Am J Obstet Gynecol. 2015;213(2):212. e211–8.

    Article  CAS  Google Scholar 

  75. Bay B, Ingerslev HJ, Lemmen JG, Degn B, Rasmussen IA, Kesmodel US. Preimplantation genetic diagnosis: a national multicenter obstetric and neonatal follow-up study. Fertil Steril. 2016;106(6):1363.

    Article  PubMed  Google Scholar 

  76. DoPierala AL, Bhatta S, Raja EA, Bhattacharya S, Bhattacharya S. Obstetric consequences of subfertility: a retrospective cohort study. BJOG. 2016;123(8):1320–8.

    Article  CAS  PubMed  Google Scholar 

  77. Nejdet S, Bergh C, Kallen K, Wennerholm UB, Thurin-Kjellberg A. High risks of maternal and perinatal complications in singletons born after oocyte donation. Acta Obstet Gynecol Scand. 2016;95(8):879–86.

    Article  PubMed  Google Scholar 

  78. Zhu LL, Zhang Y, Liu YF, Zhang RJ, Wu YQ, Huang Y, Liu F, Li MG, Sun SJ, Xing LF, et al. Maternal and live-birth outcomes of pregnancies following assisted reproductive technology: a retrospective cohort study. Sci Rep. 2016;6:35141.

  79. Vikstrom J, Josefsson A, Hammar M, Bladh M, Sydsjo G. Risk of postpartum psychosis after IVF treatment: a nationwide case-control study. Hum Reprod. 2017;32(1):139–46.

    PubMed  Google Scholar 

  80. Ben-Yaakov RD, Kessous R, Shoham-Vardi I, Sergienko R, Pariente G, Sheiner E. Fertility treatments in women who become pregnant and carried to viability, and the risk for long-term maternal cardiovascular morbidity. Am J Perinatol. 2016;33(14):1388–93.

    Article  PubMed  Google Scholar 

  81. Sun L, Zou G, Wei X, Chen Y, Zhang J, Okun N, Duan T. Clinical outcomes after assisted reproductive technology in twin pregnancies: chorionicity-based comparison. Sci Rep. 2016;6:26869.

  82. Rizzo G, Aiello E, Pietrolucci ME, Arduini D. Are there differences in placental volume and uterine artery Doppler in pregnancies resulting from the transfer of fresh versus frozen-thawed embryos through in vitro fertilization. Reprod Sci. 2016;23(10):1381–6.

    Article  PubMed  Google Scholar 

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This research did not receive any specific grant from any agency in the public, commercial, or not-for-profit sector. Esmaeil Khedmati Morasae is part-funded by the National Institute for Health Research Collaboration for Leadership in Applied Health Research and Care, North West Coast (NIHR CLAHRC NWC). The views expressed are those of the author and not necessary those of the NHS, NIHR or Department of Health.

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SM, ROS, and AAH conceived the study. MM, PA, BN, and AA collected the data. All of the authors contributed equally to the writing of the manuscript. AAH and EKM analyzed the data, and all authors revised the manuscript and approved the final version.

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Correspondence to Saman Maroufizadeh.

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Almasi-Hashiani, A., Omani-Samani, R., Mohammadi, M. et al. Assisted reproductive technology and the risk of preeclampsia: an updated systematic review and meta-analysis. BMC Pregnancy Childbirth 19, 149 (2019).

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