Skip to main content


  • Research article
  • Open Access
  • Open Peer Review

Vitamin D status in mothers and their newborns in Iran

  • 1,
  • 1,
  • 1,
  • 1,
  • 1 and
  • 1Email author
BMC Pregnancy and Childbirth20077:1

  • Received: 13 July 2006
  • Accepted: 12 February 2007
  • Published:
Open Peer Review reports



Adequate vitamin D concentrations during pregnancy are necessary to neonatal calcium homeostasis, bone maturation and mineralization. The aim of study is to evaluate serum vitamin D concentrations in mothers and their newborns and effect of vitamin D deficiency on pregnancy outcomes.


552 pregnant women were recruited from Tehran University educating hospitals in the winter of 2002. Maternal and cord blood samples were taken at delivery. The serum was assayed for 25-hydroxyvitamin D3, calcium, phosphorus and parathyroid hormone.


The prevalence of vitamin D deficiency in maternal and cord blood samples were 66.8% and 93.3%, respectively (<35 nmol/l). There was significant correlation between maternal and cord blood serum concentrations of vitamin D. In mothers with vitamin D deficiency, cord blood vitamin D concentrations was lower than those from normal mothers (P = .001). Also, a significant direct correlation was seen between maternal vitamin D intake and weight gain during pregnancy.


Consideration to adequate calcium and vitamin D intake during pregnancy is essential. Furthermore, we think it is necessary to reconsider the recommendation for vitamin D supplementation for women during pregnancy.


  • Cord Blood
  • Head Circumference
  • Serum Vitamin
  • Cord Blood Sample
  • Maternal Weight Gain


Significant changes occur in maternal calcium metabolism during pregnancy. These changes occur to obtain the extra calcium needed for fetal skeletal growth [1, 2]. Serum concentrations of 1,25(OH)2D increase 50–100% over the non-pregnant state during the second trimester, and by 100% during the third trimester [4, 5]. The mechanism underlying the increased serum 1,25(OH)2D concentrations during pregnancy is not clear. PTH, which is usually considered the stimulus for increased renal hydroxylation of 25(OH)D to 1,25(OH)2D, has not been shown to be increased during pregnancy [46], and it has been speculated that the 1,25(OH)2D present in the maternal circulation may be of placental origin [7]. It is generally accepted that maternal vitamin D status during pregnancy reflects the maternal and neonatal calcium homeostasis [813].

Several studies have identified a surprisingly high prevalence of VDD in pregnant women in temperate regions such as the United Kingdom [11, 1417] and Norway [18], and even in sunny countries such as Pakistan [1], India [19], and Saudi Arabia [20]. In a pilot study in Iran, 80% of pregnant women had 25-hydroxyvitamin D [25(OH)D] concentrations less than 25 nmol/l. The mean cord serum 25(OH)D concentration was very low (4.9 +/- 9.4 nmol/l), and that from infants whose mothers had hypovitaminosis D were almost undetectable (1.2 +/- 1.2 nmol/l) [21]. Also, the prevalence of severe, moderate and mild vitamin D deficiency was 9.5%, 57.6% and 14.2%, respectively in a general Iranian population [22].

Several researches subsequently reported that infants of mothers with low vitamin D intake during pregnancy had low serum calcium concentrations in cord blood or during the first week of life [8, 9, 23]. Also, it may be possible that maternal vitamin D status affects fetal growth and bone development [24, 25].

The aim of this study was to determine vitamin D status among pregnant women and their newborns. Also, we evaluated the effects of vitamin D status on pregnancy outcome.


As a cross-sectional study, 567 pregnant women were recruited from Tehran University educating hospitals in the winter of 2002. After taking informal consent, 15 pregnant women were excluded from the study.

Blood samples were drawn and centrifuged for 30 minutes in the delivery room. Samples were frozen at -80 degrees centigrade in the Hormone Laboratory of the Endocrinology & Metabolism Research Center (EMRC).

The study protocol was approved by the research ethics committee of EMRC, the Medical Ethics Research Center and the ethics committee of the Iran Ministry of Health and Medical Education.


Subjects were healthy pregnant women and their newborns. Exclusion criteria were those with a known history or evidence of rheumatoid arthritis, thyroid, parathyroid or adrenal diseases, hepatic or renal failure, metabolic bone disease, type 1 diabetes mellitus, malabsorption, or medications influencing bone, vitamin D or calcium metabolism. Any kind of calcium or vitamin D supplementation in the current pregnancy was also noted.


Serum concentration of 25-hydroxy vitamin D3 was measured by a radioimmunoassay (RIA) method using a Biosource kit (Biosource Europe S.A, Belgium); intra- and inter-assay coefficients of variation (CV) were 5.2% and 7.5%, respectively (normal range: 2.5–75 ng/mL). Serum PTH was also detected using a Biosource kit (Biosource Europe S.A; normal range: 13–66 pg/ml), with intra- and inter-assay CV of 6.3% and 5.7%, respectively. Alkaline phosphatase was measured by enzymatic colorimetry using a Pars Azmoon kit (Pars Azmoon, Iran); Serum calcium and phosphorous were both measured by colorimetery using the Kavoshyar enzyme kit (Kavoshyar, Iran) and Sheem enzyme kit (Sheem enzyme, Iran), respectively.

Daily intake of dietary calcium and vitamin D was calculated from a food frequency questionnaire that was approved by the nutrition group of EMRC.

Newborn anthropometric measurements were performed at birth, with recording of head circumference to the nearest 1 mm (paper tape measure), length to the nearest 1 mm (Rollametre; Childhood Growth Foundation, London, United Kingdom), weight to the nearest 10 g (Seca, Birmingham, United Kingdom), and the largest diameter of anterior and posterior fontanels to the nearest 1 mm (paper tape measure).

Mothers' weight gains during pregnancy and infants' weights and gestational ages at birth were obtained from the medical records. LBW infants were classified as those whose weights were below 2500 g.

Statistical analysis

We classified serum levels of 25(OH)D into four groups for deficiency status (<12.5 as severe, 12.5–24.9 as moderate, 25–34.9 as mild, and >34.9 nmol/L as normal). Data were analyzed using SPSS software, version 11.5. The student's T-test was used to compare the differences between the means of variables. The chi-square test was used to compare the frequency of variables. Pearson correlation was used to investigate correlation between two variables. In all tests, the level of significance was 0.05.


In total, 552 mothers participated in this study. Maternal and newborn characteristics are summarized in Table 1. There was no significant correlation between maternal serum vitamin D concentration with age (P = 0.2) or BMI (P = 0.2).
Table 1

Maternal and newborn characteristics

Gestational age (week)

38.38 ± 3.31*


1.02 ± 1.51*


1.84 ± 1.91*

Birth height (cm)

50.02 ± 3.16*

Birth weight (kg)

3.19 ± 0.45*

Preterm labor (<37)


LBW (<2500)


APGAR score

8.7 ± 0.62*

Cesarean section


Adequate vitamin D intake


Adequate calcium intake


* Mean ± 2SD

The mean maternal serum 25(OH)D concentration was 27.8 ± 21.71 nmol/l, and the prevalence of vitamin D deficiency in mothers was 66.8% (<35nmol/l), while only 3.4% of pregnancies had serum levels of 25(OH)D higher than 80 nmol/l. Of note, the prevalence of severe, moderate, and mild vitamin D deficiency in newborns was 33.8%, 51.8%, and 7.7%, respectively.

Levels of maternal and cord blood biochemical markers are summarized in Table 2. Serum vitamin D concentrations and ALP were inversely correlated with each other in mothers (r = -.265, p = .001), and there was also an inverse correlation between serum vitamin D concentrations and PTH in mothers (r = -.18, P = .002). No correlation was seen between serum vitamin D concentrations and calcium or phosphorus.
Table 2

Levels of maternal and cord blood biochemical markers




25 (OH) D (nmol/l)

27.82 ± 21.71*

18.1 ± 11.6*

Ca (mmol/l)

9.03 ± 1.3*

10.58 ± 1.38*


539.47 ± 274.32*

520.78 ± 347.6*

PTH (pg/ml)

23 ± 19.7*

16 ± 14.34*

* Mean ± 2SD

43.5 percent of women had received adequate vitamin D and calcium intake during pregnancy. No significant correlation was observed between maternal and cord blood serum vitamin D levels with calcium and vitamin D intake (P = 0.1). Accordingly, maternal serum vitamin D strongly correlated with cord blood vitamin D levels (p = 0.0001, r = 0.706). There was also a significant correlation between maternal and cord blood calcium levels (r = 0.23, P = 0.002).

Significant inverse correlations were seen between cord blood levels of vitamin D and ALP (r = -0.29, p = 0.0001) and PTH (r = -0.158, p = 0.03). In addition, a significant correlation was observed between cord blood calcium concentrations and ALP (P = .04, r = .145), and also between PTH and ALP in cord blood (P = 0.006, r = 0.2). There was no significant correlation between maternal and cord blood PTH concentrations (P = 0.1).

Among the newborns of mothers who had vitamin D deficiency, vitamin D concentrations in cord blood were lower than newborns of normal mothers (15.6 ± 7.2 vs. 20.2 ± 14.9, p = .009). Among the newborns with severe vitamin D deficiency (<12.5 ng/ml), serum ALP was higher (331.4 ± 95 compared with 606.9 ± 270.3, p = 0.001), while there was not a significant different in PTH or calcium in cord blood.

Anthropometric measurements

A significant direct correlation was seen between maternal vitamin D intake and weight gain during pregnancy (r = .11, p = .01), and also between maternal ALP and birth weight (r = .014, p = .02). But there was no significant effect of maternal vitamin D deficiency on weight gain during pregnancy, and there was no correlation between maternal calcium and weight gain.

No significant correlation was observed between maternal and cord blood vitamin D concentration and the newborns' characteristics (weight, height, head circumference, posterior and anterior fontanel diameter and APGAR). Conversely, maternal PTH directly correlated with posterior (r = .21, p= .001) and anterior (r = .18, p = .005) fontanel diameter. Furthermore, no significant correlation was seen between cord blood calcium and newborns' characteristics. In contrast, the diameter of the posterior fontanel was significantly wider in newborns of mothers with vitamin D deficiency than those of normal mothers. Finally, no significant correlation was observed between LBW and cord blood or maternal vitamin D deficiency.


Our findings reveal a high prevalence of vitamin D deficiency among pregnant women and newborns. Almost two in three mothers had vitamin D deficiency (VDD), while one in ten newborns had normal vitamin D. Vitamin D deficiency in pregnant women and newborns has been reported in several studies [18, 2628]. This prevalence is reported based on an old definition of vitamin D deficiency, and many investigators now define deficiency as <80 nM (32 ng/mL) circulating 25(OH)D/L [29, 30]. Based on this cut-off, only 4% of pregnancies in our study had normal serum vitamin D concentrations. It seems most studies define the cut-off point for vitamin D and calcium intake based on serum vitamin D and calcium concentrations. Thus new cut off point definition based on outcomes may be more suitable [12, 23, 3134]

Our results add to the evidence that cord blood 25(OH)D strongly correlated with maternal values, as reported by several studies [15, 19, 23, 26, 27, 35, 36]. In this study we found a significant negative correlation between maternal vitamin D and PTH concentrations, in agreement with the other studies [19, 3537]. Studies have shown that long-term vitamin D deficiency can result in increased PTH concentrations and decreased serum 1,25(OH)2D concentrations, which leads to osteomalacia [38]. In contrast, Datta et al. recently screened 160 pregnant women from ethnic minority groups in South Wales and found that 50% had low serum 25(OH)D concentrations, whereas PTH concentrations were within the normal range for 81% of the women with low 25(OH)D concentrations [11].

In the current study there was a weak inverse correlation between maternal serum ALP and vitamin D concentrations, which confirms the results of other studies. Brooke et al. [15] reported elevation of ALP in 20% of Asian subjects from the United Kingdom with serum 25(OH)D concentrations below 25 nmol/L. Rab and Baseer [42] from Pakistan reported elevated total ALP in 26% of pregnant women with low daily vitamin D intake, but serum 25(OH)D was not measured. Marya et al. [9] from India reported elevated ALP in 13% of their pregnant subjects who were not receiving vitamin D supplementation, whereas none of the subjects supplemented with vitamin D had elevated ALP.

Our results indicated that newborns of mothers with an adequate intake of vitamin D had higher cord blood vitamin D. Similarly, Delvin et al. assessed the effects of supplementation with 1000 IU/d vitamin D compared to placebo; in the treatment group, the cord blood vitamin D concentration was 45 nmol/l, three times which of the control group [39].

In this study, among the newborns with severe vitamin D deficiency, serum PTH was found to be significantly high, although there was no significant correlation between serum vitamin D levels and PTH in cord blood, consistent with other of studies [4042]. Furthermore, Okonofual et al. performed a study comparing Asian (with inadequate vitamin D intake) and white (with adequate vitamin D intake) mothers, and found lower serum 25(OH)D concentrations and higher PTH concentrations in cord blood samples from Asian neonates compared with white neonates [10].

In our study, there was a significant correlation between the newborns' vitamin D and calcium concentrations. Some studies have shown that maternal vitamin D status may influence placental calcium transfer [10, 43]. Namgung et al, in a study of Korean newborn infants born in the winter, found that 97% had markedly lower serum 25(OH)D concentrations. This may reflect decreased synthesis in the fetal kidney associated with limited 25(OH)D substrate availability, and theoretically could be a factor affecting placental calcium transfer and fetal bone mineralization [42]. Consequently, most studies report that maternal vitamin D concentration plays a crucial role in neonatal and maternal calcium homeostasis, and that infants of mothers with low vitamin D intake during pregnancy had low serum calcium concentrations in cord blood or during the first week of life [8, 9, 23].

In our study, there was no significant correlation between maternal calcium or vitamin D with maternal weight gain during pregnancy or birth weight. Also, we didn't find any significant correlation between LBW with cord blood and maternal vitamin D deficiency. A few studies showed that maternal vitamin D deficiency might affect maternal weight gain or fetal growth. Two studies by Marya et al. [9, 13] revealed a beneficial effect of vitamin D supply on birth weight, whereas others did not [28, 29, 33]. Also, Marya et al. [13] found no effect of maternal vitamin D supplementation on weight gain, while Brooke et al. observed less weight gain in the pregnancies without supplementation compared with the supplementation group [44].

Our findings showed that the diameter of fontanel was significantly wider in newborns of mothers with vitamin D deficiency than newborns of normal mothers. In follow-up assessments by Brook et al., neonates in the placebo group also had a greater fontanel area than did the supplemented group [15]. Also, in follow-up assessments by Maxwell et al., an increase in the size of the fontanel was noted among the infants of the control mothers vs. those of the treatment mothers [45]. Another study of 256 term infants conducted in China also found possible evidence for a relationship between maternal vitamin D deficiency and impaired fetal bone ossification [46]. Although Brooke et al. did not find an association between craniotabes and vitamin D status; they concluded that infants of mothers who received placebo had a larger fontanel than infants of mothers were treated with vitamin D, which is consistent with impaired ossification of the skull. In a recent study, there was a negative correlation between maternal vitamin D concentrations and the largest diameter of the inferior fontanel area [15].

In our study, there was no significant effect of maternal vitamin D deficiency on weight gain during pregnancy. Furthermore, there was no significant correlation between maternal vitamin D deficiency with the newborn's weight, height, or head circumference. In follow-up assessments by Shefras et al., there was a significant association between vitamin D received and head circumference, which remained after adjustment for confounding factors [47]. Also, Marya et al. reported infants of mothers who received vitamin D had a greater head circumference compared with infants of mothers who did not receive vitamin D [13]. The cut-off point for vitamin D deficiency may have an influence on detectable changes in pregnancy outcomes.


We conclude that vitamin D deficiency is frequent in mothers and newborns, and correlates with inadequate vitamin D and calcium intake. Because of this, vitamin D supplements have an important role in pregnant women in decreasing the risk of subsequent complications.



The study was financially supported by a grant from the Deputy of Health, Iranian Ministry of Health and Medical Education. We would also like to thank all of the personnel of the Iranian Multi-center Osteoporosis Study (IMOS) and all the individuals who took part in the study.

Authors’ Affiliations

Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, 5th Floor, Shariati Hospital, North Kargar Avenue, Tehran, 14114, Iran


  1. Widdowson EM: Changes in body composition during growth. Scientific foundations of pediatrics. Edited by: Davis JA, Dobbings J. 1981, London: Wm Heinemann Medical Books, 330-42Google Scholar
  2. Givens MH, Macy IC: The chemical composition of the human fetus. J Biol Chem. 1933, 102: 7-17.Google Scholar
  3. Whitehead M, Lane G, Young O, Campbell S, Abeyasekera G, Hillyard CJ, MacIntyre I, Phang KG, Stevenson JC: Interrelations of calcium-regulating hormones during normal pregnancy. Br Med J. 1981, 283: 102-2.View ArticleGoogle Scholar
  4. Cross NA, Hillman LS, Allen SH, Krause GF, Vieira NE: Calcium homeostasis and bone metabolism during pregnancy, lactation, and postweaning: a longitudinal study. Am J Clin Nutr. 1995, 61: 514-23.PubMedGoogle Scholar
  5. Ritchie LD, Fung EB, Halloran BP, Turnlund JR, Van Loan MD, Cann CE, King JC: A longitudinal study of calcium homeostasis during human pregnancy and lactation and after resumption of menses. Am J Clin Nutr. 1998, 67: 693-701.PubMedGoogle Scholar
  6. Bezerra FF, Laboissiere FP, King JC, Donangelo CM: Pregnancy and lactation affect markers of calcium and bone metabolism differently in adolescent and adult women with low calcium intakes. J Nutr. 2002, 132: 2183-7.PubMedGoogle Scholar
  7. Seki K, Makimura N, Mitsui C, Hirata J, Nagata I: Calcium-regulating hormones and osteocalcin levels during pregnancy: a longitudinal study. Am J Obstet Gynecol. 1991, 164: 1248-52.View ArticlePubMedGoogle Scholar
  8. Congdon P, Horsman A, Kirby PA, Dibble J, Bashir T: Mineral content of the forearms of babies born to Asian and white mothers. BMJ. 1983, 286: 1234-5.Google Scholar
  9. Marya RK, Rathee S, Lata V, Mudgil S: Effects of vitamin D supplementation in pregnancy. Gynecol Obstet Invest. 1981, 12: 155-61.View ArticlePubMedGoogle Scholar
  10. Okonofua F, Menon RK, Houlder S, Thomas M, Robinson D, O'Brien S, Dandona P: Calcium, vitamin D and parathyroid hormone relationships in pregnant Caucasian and Asian women and their neonates. Ann Clin Biochem. 1987, 24: 22-8.View ArticlePubMedGoogle Scholar
  11. Datta S, Alfaham M, Davies DP, Dunstan F, Woodhead S, Evans J, Richards B: Vitamin D deficiency in pregnant women from a non-European ethnic minority population: an interventional study. Br J Obstetr Gynaecol. 2002, 109: 905-8.Google Scholar
  12. Cockburn F, Belton NR, Purvis RJ, Giles MM, Brown JK, Turner TL, Wilkinson EM, Forfar JO, Barrie WJ, McKay GS, Pocock SJ: Maternal vitamin D intake and mineral metabolism in mothers and their newborn infants. Br Med J. 1980, 231: 1-10.Google Scholar
  13. Marya RK, Rathee S, Dua V, Sangwan K: Effect of vitamin D supplementation during pregnancy on foetal growth. Indian J Med Res. 1988, 88: 488-92.PubMedGoogle Scholar
  14. Heckmatt JZ, Pocock M, Davies AEJ, McMurray J, Isherwood DM: 25-hydroxyvitamin D in pregnant Asian women and their babies. Lancet. 1979, 1: 546-9. 10.1016/S0140-6736(79)91612-X.View ArticleGoogle Scholar
  15. Brooke OG, Brown IRF, Cleeve HJW, Sood A: Observations on the vitamin D state of pregnant Asian women in London. Br J Obstet Gynaecol. 1981, 88: 18-26.View ArticlePubMedGoogle Scholar
  16. Howarth AT: Biochemical indices of osteomalacia in pregnant Asian immigrants in Britain. J Clin Pathol. 1976, 29: 981-3.View ArticlePubMedPubMed CentralGoogle Scholar
  17. Dent CE, Gupta MM: Plasma 25-hydroxyvitamin-D levels during pregnancy in Caucasians and in vegetarian and non-vegetarian Asians. Lancet. 1975, 2: 1057-60. 10.1016/S0140-6736(75)90430-4.View ArticlePubMedGoogle Scholar
  18. Henriksen C, Brunvand L, Stoltenberg C, Trygg K, Haug E, Pedersen JI: Diet and vitaminDstatus among pregnant Pakistani women in Oslo. Eur J Clin Nutr. 1995, 49: 211-8.PubMedGoogle Scholar
  19. Sachan A, Gupta R, Das V, Agarwal A, Awasth PK, Bhatia v: High prevalence of vitamin D deficiency among pregnant women and their newborns in northern India. Am J Clin Nutr. 2005, 81: 1060-4.PubMedGoogle Scholar
  20. Serenius F, Elidrissy AT, Dandona P: Vitamin D nutrition in pregnant women at term and in newly born babies in Saudi Arabia. J Clin Pathol. 1984, 37: 444-7.View ArticlePubMedPubMed CentralGoogle Scholar
  21. Bassir M, Laborie S, Lapillonne A, Claris O, Chappuis MC, Salle BL: Vitamin D deficiency in Iranian mothers and their neonates: a pilot study. Acta paediatr. 2001, 90: 577-9.View ArticlePubMedGoogle Scholar
  22. Hashemipour S, Larijani B, Adibi H, Javadi E, Sedaghat M, Pajouhi M, Soltani A, Shafaei A, Hamidi Z, Fard A, Hossein-Nezhad A, Booya F: Vitamin D deficiency and causative factors in the population of Tehran. BMC Public Health. 2004, 25 (4): 38-10.1186/1471-2458-4-38.View ArticleGoogle Scholar
  23. Paunier L, Lacourt G, Pilloud P, Schlaeppi P, Sizonenko PC: 25-HydroxyvitaminDand calcium levels in maternal, cord and infant serum in relation to maternal vitamin D intake. Helv Paediatr Acta. 1978, 33: 95-103.PubMedGoogle Scholar
  24. Moncrieff M, Fadahunsi TO: Congenital rickets due to maternal vitamin D deficiency. Archives of Disease in Childhood. 1974, 49: 810-811.View ArticlePubMedPubMed CentralGoogle Scholar
  25. Nozza JM, Rodda CP: Vitamin D deficiency in mothers of infants with rickets. Med J Aust. 2001, 3: 253-5.Google Scholar
  26. Weiler H, Fitzpatrick-Wong S, Veitch R, Kovacs H, Schellenberg J, McCloy U, Yuen CK: Vitamin D deficiency and whole-body and femur bone mass relative to weight in healthy newborns. CMAJ. 2005, 172: 757-61.View ArticlePubMedPubMed CentralGoogle Scholar
  27. Taha SH, Dost SM, Sedrani SH: 25OHD and total Ca: extraordinary low plasma concentrations in Saud: mothers and their neonates. Res. 1984, 18: 739-41.Google Scholar
  28. Pawley N, Bishop NJ: Prenatal and infant predictors of bone health: the influence of vitamin D. Am J Clin Nutr. 2004, 1748-51. Suppl 80Google Scholar
  29. Hollis BW: Circulating 25-hydroxyvitamin D levels indicative of vitamin D sufficiency: implications for establishing a new effective dietary intake recommendation for vitamin D. J Nutr. 2005, 135: 317-22.PubMedGoogle Scholar
  30. Dawson-Hughes B, Heaney RP, Holick MF, Lips P, Meunier PJ, Vieth R: Vitamin D round table. Nutritional aspects of osteoporosis. Edited by: Burckhardt P, Dawson-Hughes B, Heaney R. 2004, Burlington, MA: Elsevier Science and Technology Books, 263-70. 2Google Scholar
  31. Hollis BW, Wagner CL: Assessment of dietary vitamin D requirements during pregnancy and Lactation. Am J Clin Nutr. 2004, 79: 717-26.PubMedGoogle Scholar
  32. Vieth R, Chan PCR, MacFarlane GD: Efficiency and safety of vitamin D3 intake exceeding the lowest observed adverse effect level (LOAEL). Am J Clin Nutr. 2001, 73: 288-94.PubMedGoogle Scholar
  33. Heaney RP, Davies KM, Chen TC, Holick MF, Barger-Lux MJ: Human serum 25-hydroxycholecalciferol response to extended oral dosing with cholecalciferol. Am J Clin Nutr. 2003, 77: 204-10.PubMedGoogle Scholar
  34. Hollis BW, Wagner CL: Vitamin D requirements during lactation: high-dose maternal supplementation as therapy to prevent hypovitaminosis Din both mother and nursing infant. Am J Clin Nutr. 2004, 1752-8. Suppl 80Google Scholar
  35. Sowers M, Crutchfield M, Jannausch M, Updike S, Corton G: A prospective evaluation of bone mineral change in pregnancy. Obstet Gynecol. 1991, 77: 841-5.PubMedGoogle Scholar
  36. Mallet E, Gugi B, Brunelle P, Henocq A, Basuyau JP, Lemeur H: Vitamin D supplementation in pregnancy: a controlled trial of two methods. Obstet Gynecol. 1986, 68: 300-4.View ArticlePubMedGoogle Scholar
  37. Brunvand L, Shah SS, Bergstrom S, Haug E: Vitamin D deficiency in pregnancy is not associated with obstructed labor. A study among Pakistani women in Karachi. Acta Obstet Gynecol Scand. 1998, 77: 303-306. 10.1034/j.1600-0412.1998.770309.x.View ArticlePubMedGoogle Scholar
  38. Specker B: Vitamin D requirements during pregnancy. Am J Clin Nutr. 2004, 1740-7. Suppl 80Google Scholar
  39. Delvin EE, Salle BL, Glorieux FH, Adeleine P, David LS: Vitamin D supplementation during pregnancy: effect on neonatal calcium homeostasis. J Pediatr. 1986, 109: 328-34. 10.1016/S0022-3476(86)80396-1.View ArticlePubMedGoogle Scholar
  40. Black AJ, Topping J, Durham B, Farquharson RG, Fraser WD: A detailed assessment of alterations in bone turnover, calcium homeostasis and bone density in normal pregnancy. J Bone Miner Res. 2000, 15: 557-63. 10.1359/jbmr.2000.15.3.557.View ArticlePubMedGoogle Scholar
  41. Yoon BK, Lee JW, Choi DS, Roh CR, Lee JH: Changes in biochemical bone markers during pregnancy and puerperium. J Korean Med Sci. 2000, 15: 189-93.View ArticlePubMedPubMed CentralGoogle Scholar
  42. Namgung R, Tsang RC, Lee C, Han DG, Ho ML, Sierra RI: Low total body bone mineral content and high bone resorption in Korean winter-born versus summer-born newborn infants. Journal of Pediatrics. 1998, 132: 421-425. 10.1016/S0022-3476(98)70013-7.View ArticlePubMedGoogle Scholar
  43. Durand D, Braithwaite GD, Barlet JP: The effect of 1-hydroxycholecalciferol on the placental transfer of calcium and phosphate in sheep. British Journal of Nutrition. 1983, 49: 475-480. 10.1079/BJN19830056.View ArticlePubMedGoogle Scholar
  44. Brooke DG, Brown IRF, Bone CDM: Vitamin D supplements in pregnant Asian women: effects on calcium status and fetal growth. Br Med J. 1980, 280: 751-4.View ArticlePubMedPubMed CentralGoogle Scholar
  45. Maxwell JD, Ang L, Brooke OG, Brown IR: Vitamin D supplements enhance weight gain and nutritional status in pregnant Asians. Br J Obstet Gynaecol. 1981, 88: 987-91.View ArticlePubMedGoogle Scholar
  46. Specker BL, Ho ML, Oestreich A, Yin TA, Shui QM, Chen XC, Tsang RC: Prospective study of vitamin D supplementation and rickets in China. Journal of Pediatrics. 1992, 120: 733-739. 10.1016/S0022-3476(05)80236-7.View ArticlePubMedGoogle Scholar
  47. Shefras J, Farquharson R: Bone density studies in pregnant women receiving heparin. Eur J Obstet Gynecol Reprod Biol. 1996, 65: 171-4. 10.1016/0301-2115(95)02358-5.View ArticlePubMedGoogle Scholar
  48. Pre-publication history

    1. The pre-publication history for this paper can be accessed here:


© Maghbooli et al; licensee BioMed Central Ltd. 2007

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.