Fig. 6
Interferon production by peripheral blood mononuclear cells (PBMCs) upon TLR7/TLR8 or TLR3 stimulation. A Peripheral blood samples were collected from pregnant (n = 20, indicated in red) and non-pregnant (n = 20, indicated in blue) women to isolate PBMCs for in vitro stimulation with R848 or Poly(I:C) (HMW) VacciGrade™ [Poly(I:C)]. Type-I (IFN-α2a, -β), Type-II (IFN-γ) and Type-III (IL-29/IFN-λ1) interferon concentrations were then determined in culture supernatants. B-E Log10-transformed concentrations of (B) IFN-α2a, (C) IFN-β, (D) IFN-γ, and (E) IL-29/IFN-λ1 in culture supernatants of PBMCs from pregnant (red symbols) and non-pregnant (blue symbols) women in response to R848 (solid circles) or control (open circles). F-I Log10-transformed concentrations of (F) IFN-α2a, (G) IFN-β, (H) IFN-γ, and (I) IL-29/IFN-λ1 in culture supernatants of PBMCs from pregnant (red symbols) and non-pregnant (blue symbols) women in response to Poly(I:C) (solid circles) or control (open circles). Dotted lines indicate the detection limit of each analyte. **p < 0.01, ***p < 0.001. ( +) Stimulated, (-) Control