Fig. 5
Monocyte response to TLR9 stimulation. A Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of pregnant (n = 20) and non-pregnant (n = 20) women with and without ODN2216 stimulation for 24 h. Flow cytometry was performed to evaluate the total monocyte subsets. B Proportions of monocyte subsets in pregnant (red) and non-pregnant (blue) women with ODN2216 stimulation. C Heatmap representation of the differences in proportions of monocyte subsets from pregnant (red symbols) and non-pregnant (blue symbols) women following ODN2216 stimulation. Asterisks indicate statistically significant differences between the indicated groups. D-G Frequencies of (D) classical monocytes (CD14hiCD16−), (E) intermediate monocytes (CD14hiCD16+), (F) non-classical monocytes (CD14loCD16+), and (G) CD14loCD16− monocytes in pregnant (red) and non-pregnant (blue) women following ODN2216 stimulation (solid circles) or control (open circles). ( +) Stimulated; (-) Control