Fig. 3
Monocyte response to TLR3 stimulation. A Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of pregnant (n = 20) and non-pregnant (n = 20) women and stimulated with Poly(I:C) (HMW) Vaccigrade™ (poly(I:C); TLR3 agonist) or Poly(I:C) (HMW)LyoVec™ (poly(I:C)/LyoVec; RIG-I/MDA5 agonist) for 24 h. Flow cytometry was performed to phenotype monocytes. B Proportions of monocyte subsets in pregnant (red) and non-pregnant (blue) women with and without Poly(I:C) (HMW) Vaccigrade™ stimulation. C Heatmap representation of the differences in proportions of monocyte subsets from pregnant (red symbols) and non-pregnant (blue symbols) following Poly(I:C) (HMW) Vaccigrade™ stimulation. Asterisks indicate statistically significant differences between the indicated groups. (D-M) Frequencies of (D) classical monocytes (CD14hiCD16−), (E) CD14loCD16− monocytes, (F) intermediate monocytes (CD14hiCD16+), (G) non-classical monocytes (CD14loCD16+), (H) CD147+ monocytes, (I) CD162+ monocytes, (J) CD182−CD181+ monocytes, (K) CD182+CD181+ monocytes, (L) CD182+CD181− monocytes, and (M) CD182−CD181− monocytes in pregnant (red) and non-pregnant (blue) women following Poly(I:C) stimulation (solid circles) or control (open circles). *p < 0.05; **p < 0.01; ***p < 0.001. ( +) Stimulated; (-) Control