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Fig. 3 | BMC Pregnancy and Childbirth

Fig. 3

From: Acute endometriosis-related sigmoid perforation in pregnancy- case report

Fig. 3

pathology findings: Immunohistochemical analysis was performed on formalin-fixed, paraffin-embedded tissue sections on an automated immunostainer (Ventana Medical Systems, Tucson, AZ, USA). Representative Slides were stained with the following antibodies: BerEP4 (DAKO, Hamburg, Germany), CD10 (Novocastra, Berlin, Germany). Appropriate positive and negative controls were used to confirm the adequacy of the staining. Images were acquired with an Axioskop 2 plus Zeiss microscope equipped with a Jenoptik (Laser Optik System, Jena, Germany) ProgRes C10 plus camera and software. Objectives Plan-Neofluar used were: 1.25/0.035, × 2.5/0.075, × 10/0.30, × 20/0.50 and × 40/0.75. A Endometriosis with decidualized endometrioid stroma infiltrating large bowel wall, mucosa and submucosa (25 x). B Dezidualized endometrioid stroma in mucosa and submucosa (200x). C Immunohistochemistry for CD10 demonstrating endometrioid stroma (200x). D Immunohistochmistry for epithelial marker Ber-EP4 demonstrating sparse endometrioid glands in the endometriosis (200x)

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