Table 1 Sequences of the primers used for the quantification of the target genes (F: Forward; R: Revers)
Gene | NIH Gen Bank accession No | Product length (bp) | Primer sequence 5´- 3´ |
|---|---|---|---|
STAR | NM:000,349.2 | 114 | GCCCAAGAGCATCATCAAC(F) GCTGGTCTTCAACACCTG (R) |
HSD11B1 | NM:001,206,741.1 | 180 | GCATTGTTGTCGTCTCCTCT(F) TGGCTGTTTCTGTGTCTATGAG(R) |
HSD11B2 | NM:000,196.3 | 162 | GCTGTGAACTCCTTCCCT(F) CGATGTAGTCCTTGCCGT(R) |
HSD17B2 | NM:002,153.2 | 136 | TCTGCCTGCTCATCCTGT(F) CAATCACCACCTGTCACCA(R) |
HSD17B3 | NM:000,197.1 | 138 | ATGCTTCCAAACCTTCTCCC(F) GAGACCTTTCTGCCTTGATTCC(R) |
HSD17B5 | NM:001,253,908.1 | 151 | CTCCAGAGGTTCCGAGAA(F) CTCTTCACACTGCCATCTG(R) |
HSD17B7 | NM:001,304,512.1 | 112 | TCATCTGTGTTTGGCGTG(F) GTTGCTGACATCCACCTG(R) |
HSD17B12 | NM:016,142.2 | 113 | CCTACCTAGCCCTGCGTATT(F) ACCTGTGACAACTGCCCA(R) |
HSD3B1 | NM:000,862.2 | 185 | CTTGGTGAAGGAGAAGGAGC(F) AGGCGGTGTGGATGATGA(R) |
HSD3B2 | NM:000,198.3 | 173 | TGCCAGTCTTCATCTACACC(F) TAGATTCCACCCATTAGCCG(R) |
CYP17A1 | NM:000,102.3 | 154 | GATAACCACATTCTCACCACC(F) GGCTGAAACCCACATTCTG(R) |
CYP11A1 | NM:000,781.2 | 169 | CTTCCTTTCTGTCTCAATTCCC(F) TCTACCAGATGTTCCACACC(R) |
CYP21A2 | NM:000,500.8 | 103 | TGAAGCAGGCCATAGAGAAG(F) ATGTAGTCCATCATGTCCCTC(R) |
HSD17B1 | NM:000,413.3 | 234 | TTCAGATCCATCCCAGAGC(F) TTGATGTCCCTTACGTCCAG(R) |
CYP19 | NM: 000,103.4 | 116 | AGGAGGTGACCAATGAATCG (F) CACGATAGCACTTTCGTCCA (R) |
GAPDH | NM: 001,256,799.3 | 134 | TGAGAAGTATGACAACAGCCTC(F) TGATGGCATGGACTGTGGT (R) |