Table 2 Demographic, clinical and laboratory data for the “top cycle” selected population after blastocyst transfers or cleaved embryo transfer. Analysis between pregnant and not pregnant women

Female age (y.o)

30.7 ± 2.4

31.0 ± 2.8

NS

30.4 ± 2.5

30.3 ± 2.3

NS

Female BMI (kg/m²)

25.2 ± 4.5

24.7 ± 2.1

NS

25.7 ± 4.9

24.8 ± 4.1

NS

Female smoking, %

26.7

19.5 ^e

NS

31.2

29.9 ^f

NS

Male BMI (kg/m²)

25.1 ± 3.3

25.3 ± 4.3

NS

25.3 ± 4.0

25.2 ± 4.2

NS

Male smoking, %

34.2

28.3 ^g

NS

38.7

38.0 ^h

NS

Primary infertility, %

40.0 ^c

43.8 ^a

NS

61.0 ^d

66.8 ^b

NS

Indications %

Tubal disorder

16.1

15.0

NS

15.2

13.7

NS

Endometriosis

6.6

8.2

NS

7.6

6.8

NS

Ovulatory dysfunction

8.5

11.8

NS

3.8

4.8

NS

Low ovarian reserve

6.6

5.1

NS

9.1

8.5

NS

Unexplained

11.4

10.8

NS

10.6

9.7

NS

Male factor

30.4

30.4

NS

37.4

38.4

NS

Mixed

20.4

18.8

NS

16.0

17.8

NS

AMH ng/ml

3.70 ± 2.19

4.10 ± 3.60

NS

3.41 ± 1.99

3.70 ± 3.39

NS

Retrieval rank (m ± SD)

0.53 ± 0.50 ^g

0.65 ± 0.47 ^e

<  0.05

0.67 ± 0.46 ^h

0.54 ± 0.50 ^f

<  0.02

Number of cycles

105

194

131

247

Total dose Gnt (IU)

2125 ± 721

1913 ± 670

<  0.02

2203 ± 853

1965 ± 813

<  0.01

FSH use, %

34.2 ^a

39.1 ^a

NS

64.8 ^b

61.9 ^b

NS

Stimulation duration (d)

10.0 ± 1.58

10.22 ± 2.96

NS

10.1 ± 1.5

10.1 ± 2.7

NS 

Mature follicle hCG day

2.52 ± 2.25

3.11 ± 2.98 ^c

NS

2.82 ± 2.72

2.22 ± 2.00 ^d

<  0.05

Estradiol hCG day-2

1155 ± 586

1264 ± 513

NS

1142 ± 584

1235 ± 513

NS

Estradiol hCG day-1

1624 ± 630

1706 ± 593

NS

1601 ± 584

1608 ± 681

NS

Estradiol hCG day

1899 ± 573

2039 ± 628

NS

1832 ± 583

2012 ± 665

<  0.01

Progesterone hCG day-2

0.75 ± 0.21

0.76 ± 0.29

NS

0.76 ± 0.23

0.75 ± 0.28

NS

Progesterone hCG day-1

0.78 ± 0.26

0.89 ± 0.27

<  0.001

0.78 ± 0.28

0.87 ± 0.28

<  0.01

Progesterone hCG day

0.86 ± 0.21

0.99 ± 0.39

<  0.001

0.86 ± 0.21

1.00 ± 0.39

<  0.001

LH hCG day-2

2.73 ± 1.86

1.61 ± 1.39

<  0.001

2.66 ± 1.72

1.55 ± 1.31

<  0.001

LH hCG day-1

1.42 ± 0.81

1.34 ± 1.16

NS

1.51 ± 0.83

1.41 ± 1.27

NS

LH hCG day

2.15 ± 1.30

1.58 ± 1.47

<  0.001

2.25 ± 1.31

1.68 ± 1.43

<  0.001

Endometrium (mm)

9.63 ± 1.47

9.54 ± 1.78

NS

9.66 ± 1.51

9.53 ± 1.75

NS

Stimulation cancelled

5 (4.7%)

11 (5.6%)

NS

6 (4.5%)

13 (5.2%)

NS

Number oocyte retrievals

100

183

125

234

Retrieved oocytes (m ± SD)

8.73 ± 3.25

9.89 ± 3.74

<  0.01

8.16 ± 3.24

9.26 ± 3.81

<  0.01

Mature oocytes (m ± SD)

7.38 ± 2.64

7.96 ± 3.06

NS

6.85 ± 2.71

7.35 ± 3.19

NS

ICSI, %

34.6 ^a

32.9 ^a

NS

57.3 ^b

53.7 ^b

NS

2 PN (m ± SD)

5.40 ± 2.40

6.06 ± 2.68 ^c

< 0.05

4.90 ± 2.44

5.36 ± 2.87 ^d

NS

Fertilization rate, %

74.3

76.1

NS

72.5

72.9

NS

Total embryos (m ± SD)

5.18 ± 2.35

6.15 ± 2.55 ^c

< 0.01

4.94 ± 2.45

5.48 ± 2.71 ^d

NS

Cleavage rate, %

75.4

77.3

NS

73.1

74.5

NS

Blastocyst (m ± SD)

2.93 ± 1.77

3.25 ± 1.91

NS

–

–

% Blastocyst/embryo

53.5

52.7

NS

–

–

Culture failure

40 (40.0%)

75 (40.9%)

NS

50 (40.0%)

100 (42.7%)

NS

Number of transfers

60

108

75

134

Embryo transferred (m ± SD)

1.15 ± 0.34

1.13 ± 0.33

NS

1.17 ± 0.37

1.14 ± 0.35

NS