Table 1 Demographic, clinical and laboratory data for the general population after blastocyst or cleaved embryo transfer. Analysis between pregnant and not pregnant women

Female age (y.o)

33.8 ± 6.8

33.4 ± 7.8

NS

33.1 ± 7.4

33.7 ± 9.2

NS

Female BMI (kg/m²)

24.8 ± 4.5

25.0 ± 5.0

NS

25.2 ± 4.7

24.9 ± 4.2

NS

Female tobacco %

50.0 ^c

32.0 ^g

<  0.001

35.0 ^d

24.6 ^h

<  0.05

Male BMI (kg/m²)

25.1 ± 3.3

25.2 ± 3.8

NS

25.4 ± 7.3

24.8 ± 8.7

NS

Male tobacco %

51.8

33.0 ^c

<  0.001

43.8

44.0 ^d

NS

Primary infertility %

65.0

60.8

NS

59.6

62.5

NS

Indications %

Tubal disorder

16.2

17.9

NS

15.7

13.9

NS

Endometriosis

6.3

7.7

NS

7.1

6.0

NS

Ovulatory dysfunction

6.8

7.0

NS

3.5

4.6

NS

Low ovarian reserve

5.6

6.0

NS

10.7

10.7

NS

Unexplained

14.5

9.9

NS

11.4

8.9

NS

Male factor

28.7

33.6

NS

35.7

35.0

NS

Mixed

21.8

17.9

NS

15.7

21.0

NS

AMH, ng/ml

3.46 ± 2.41 ^a

3.05 ± 2.70 ^a

NS

1.95 ± 0.66 ^b

2.07 ± 1.10 ^b

NS

Retrieval rank (m ± SD)

1.05 ± 0.9

1.14 ± 0.9 ^e

NS

1.17 ± 1.0

1.34 ± 1.3 ^f

NS

Number of cycles

160

312

192

546

Total dose of Gnt (IU)

2222 ± 819 ^a

2281 ± 633 ^a

NS

2727 ± 664 ^b

3127 ± 674 ^b

<  0.001

FSH use, %

57.5

58.3

NS

65.1

63.5

NS

Stimulation duration (d)

10.06 ± 1.60

9.82 ± 1.49 ^c

NS

10.07 ± 1.79

10.18 ± 1.76 ^d

NS

Mature follicle hCG day

3.4 ± 2.0

4.1 ± 2.7 ^a

<  0.01

3.2 ± 1.8

2.5 ± 2.1 ^b

<  0.001

Estradiol hCG day-2

1161 ± 530

1269 ± 528

<  0.05

1004 ± 335

1093 ± 460

<  0.05

Estradiol hCG day-1

1677 ± 607

1750 ± 723

NS

1472 ± 398

1464 ± 675

NS

Estradiol hCG day

1973 ± 613 ^a

1997 ± 689 ^a

NS

1667 ± 531 ^b

1733 ± 722 ^b

NS

Progesterone hCG day-2

0.73 ± 0.21

0.75 ± 0.30

NS

0.65 ± 0.17

0.79 ± 0.26

<  0.001

Progesterone hCG day-1

0.78 ± 0.29

0.90 ± 0.29

<  0.001

0.75 ± 0.27

0.84 ± 0.32

<  0.01

Progesterone hCG day

0.84 ± 0.22

0.99 ± 0.36 ^a

<  0.001

0.83 ± 0.22

0.91 ± 0.31 ^b

<  0.01

LH hCG day-2

2.17 ± 1.32

1.43 ± 1.23

<  0.001

1.68 ± 1.19

1.77 ± 1.32

NS

LH hCG day-1

1.73 ± 1.19

1.34 ± 1.28

<  0.01

1.37 ± 0.76

1.88 ± 1.65

<  0.001

LH hCG day

1.80 ± 1.47

1.49 ± 1.34 ^a

<  0.05

1.61 ± 0.77

1.90 ± 1.27 ^b

<  0.01

Endometrium (mm)

9.51 ± 1.49 ^e

9.62 ± 1.99

NS

9.98 ± 2.08 ^f

9.60 ± 1.86

NS

Stimulation cancelled

6 (3.7%)

12 (3.8%)

NS

8 (4.1%)

28 (5.1%)

NS

Number oocyte retrievals

154

300

184

518

Retrieved oocytes (m ± SD)

9.10 ± 3.73 ^a

9.64 ± 4.09 ^a

NS

6.77 ± 2.68 ^b

6.43 ± 3.03 ^b

NS

Mature oocytes (m ± SD)

7.54 ± 2.73 ^a

7.84 ± 3.41 ^a

NS

5.07 ± 2.31 ^b

4.71 ± 2.47 ^b

NS

ICSI, %

57.1 ^a

57.0 ^a

NS

64.6 ^b

69.1 ^b

NS

2PN (m ± SD)

5.69 ± 2.26 ^a

5.87 ± 2.77 ^a

NS

3.56 ± 2.11 ^b

2.96 ± 1.85 ^b

<  0.01

Fertilization rate, %

75.5

74.9 ^a

NS

70.2

62.5 ^b

<  0.02

Total embryos (m ± SD)

5.75 ± 2.47 ^a

6.02 ± 2.68 ^a

NS

3.54 ± 1.86 ^b

3.08 ± 1.75 ^b

<  0.02

Cleavage rate, %

77.6

76.4 ^a

NS

69.8

65.4 ^b

NS

Blastocyst (m ± SD)

2.71 ± 1.74

2.62 ± 1.77

NS

–

–

% Blastocyst/embryo

47.2

43.6

NS

–

–

Culture failure

42 (27.2%)

76 (25.3%)

NS

54 (29.3%)

135 (26.0%)

NS

Number of transfers

112

224

130

383

Embryos transferred (m ± SD)

1.33 ± 0.94 ^c

1.30 ± 0.92 ^c

NS

1.59 ± 0.40 ^d

1.51 ± 0.89 ^d

NS