Whole-exome sequencing identifies novel mutations in ABC transporter genes associated with intrahepatic cholestasis of pregnancy disease: a case-control study

Table 1 Primes used to confirm the variants in ABC transporter genes

Order	Gene	Patient	Annealing temperature (°C)	Amplicon (bp)	Forward primer (5′-3′)	Reverse primer (5′-3′)
1	ABCA2	ICP64	56	229	CTACCCAGGTCCATCCTGAC	CCACCCACTTGTCCATCTCT
2	ABCA10	ICP35	56	158	AACCTATGCTCGCTGGTTTG	TGTTCTTACCTGGGCCATTC
3	ABCA12	ICP75	56	150	CATGGATCCGAAGTCGAAA	ACCTTTTCCCACCTGTCATC
4	ABCA13	ICP112	56	245	CAAGGACCAAGCATCATTCC	GGGAGGTGGAAGCAGAAGAT
5	ABCB4	ICP133, ICP135	50	200	ACATTCCAGGTCCTATTT	TCGAGAAGGGTAAGAAAA
6	ABCB4	ICP154	56	463	CTGTCAAAGAGGCCAACG	GCTTCTGCCCACCACTCA
7	ABCB5	ICP47	56	460	TCTTACACTAGCCATCCT	CTATTTCCACGACTTACA
8	ABCB11	ICP2	56	205	GCTATCGCCAGAGCCCTCA	TACTCCCATCCCTCCCACC
9	ABCC2	ICP79	56	368	CCTCTTACCTCCTGTGAC	GTAGACCGTGGAATTGAC
10	ABCC3	ICP93	56	189	CCCCAAACTCCTTCTCCC	TGGCATCCACCTTAGTATCA
11	ABCC9	ICP124	56	166	TCCCACAACCCATTCATCTT	GCCTCTGCCAACTTTGTAGC
12	ABCG1	ICP113	56	212	CTCACGGTGCCTCTTGACTT	GGTGTCATTCAGGCTGACCT
13	ABCG2	ICP6	56	180	GTGGCCTTGGCTTGTATGAT	GATGGCAAGGGAACAGAAAA

ISSN: 1471-2393