Fig. 4

Primary human first-trimester EEC cultures spontaneously secreted CXCL12 in vitro. Spontaneous CXCL12 secretion in the supernatants of in vitro cultured human first-trimester endometrial epithelial cells (EECs) was measured at 24, 48 and 72 h with enzyme-linked immunosorbent assay (ELISA). It was shown that human first-trimester primary cultured EECs continuously secreted CXCL12 in vitro. Data are presented as the mean ± SD (n = 3)