Fig. 1

Alpha diversity of salivary microbiota during pregancy. Samples were rarefied to an equal sequencing depth of 10,000 reads and by observed OTUs (a), Shannon’s diversity (b) and Pielou’s evenness (c) alpha diversity in 3rd trimester of pregnancy in women with gestational diabetes (GDM; n = 50) and normoglycaemic women (n = 160) is presented. Boxes represent interquartile range (IQR), with the inside line representing the median. Values within 1.5 × IQR of the first and third quartiles are represented by whiskers. Circles represent individual samples with lines connecting samples from the same individual. Student’s t-test was used to test differences between normoglycaemic and GDM pregnancies