Combination No |
|---|
Factor value | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
|---|
A or a | A | A | A | A | a | a | A | a |
B or b | B | B | b | b | B | B | B | b |
C or c | C | c | C | c | C | c | C | c |
D or d | D | D | d | d | d | d | D | D |
E or e | E | e | E | e | e | E | E | E |
F or f | F | f | f | F | F | f | f | F |
G or g | G | g | g | G | g | G | G | g |
- (A) Standardised master mix; (a) alternative master mix; (B) standardised concentration of primers; (b) lower concentrations of primers; (C) standardised concentration of probe; (c) lower concentration of probe; (D) standardised alignment temperature; (d) upper alignment temperature; (E) standardised alignment temperature; (e) lower alignment temperature; (F) equipment used for validation; (f) alternative equipment; (G) operator 1; (g) operator 2
