Fig. 2

Determination of the anticipated reportable range. a Standard curve between the value assignment (log₁₀ GE/μL) and cycle threshold, as obtained from the points used to calculate real-time polymerase chain reaction efficiency (ε). b Linear regression of the points on the standard curve between the assigned values and those obtained from measurements (log₁₀ GE/μL). Also shown is the equation obtained from the model