Figure 1

Exogenous VEGF-A is cytoprotective for trophoblasts in atmospheric oxygen conditions. A. HTR-8/SVneo (first trimester immortalised trophoblast) cells were cultured for 48 hours in SFM ± 1nM VEGF-A₁₆₅b and survival assessed by Trypan exclusion. (* = p < 0.05, Unpaired t test, n = 10). B. Cytotoxicity assessed by LDH release (n = 19, One way ANOVA, p = 0.0019, Dunnett’s Multiple Comparison Test). C. Trophoblasts were cultured in SFM ± 2nM VEGF-A₁₆₅a in 21% O₂. After 48 hours cytotoxicity determined by LDH assay. (Unpaired t test, p < 0.05, n = 13). D. In the presence of 5 mM sodium butyrate (NaBu, a cytotoxic agent), addition of 1 nM VEGF-A₁₆₅b significantly decreased the trophoblasts cytotoxicity (p < 0.05, Unpaired t test, n = 8).