The role of cervical Electrical Impedance Spectroscopy in the prediction of the course and outcome of induced labour
© Jokhi et al; licensee BioMed Central Ltd. 2009
Received: 12 March 2009
Accepted: 2 September 2009
Published: 2 September 2009
Previous work by us and others had suggested that cervical electrical impedance spectroscopy (EIS) may be predictive of the outcome of induced labour. We sought to determine which probe configuration of the EIS device is predictive of the outcome of induced labour and compare this to digital assessment by the Bishop score.
In a prospective cohort of 205 women admitted for induction of labour, we used four probes of diameter 3, 6, 9 and 12 mm connected to an impedance meter to measure cervical resistivity (CR) in Ohm.meters at 14 electrical frequencies and compared their values to digital assessment of the cervix by the Bishop score for the prediction of the outcome of induced labour. We tested the association of labour characteristics and outcomes with CR and Bishop score by stepwise multilinear regression analyses, and the accuracy of prediction of categorical clinical outcomes by analysis of the area under the curves (AUC) of derived Receiver Operator Characteristic (ROC) curves.
Of the four CR probe dimensions studied, only the 12 mm probe was predictive of any labour indices. In the frequency range 19 - 156 kHz, CR obtained with this probe was higher in women who delivered by caesarean section (CS) than those who delivered vaginally, and in labours lasting > 24 hrs. Cervical resistivity at 78.1 kHz best predicted vaginal delivery [optimal cut-off <2.25 Ohm.meter, AUC 0.66 (95% CI 0.59-0.72), sensitivity 71.0%, specificity 56.5%, LR+ 1.63, LR- 0.51, P < 0.01] and labour duration >24 hrs [optimal cut-off 2.27 Ω.m, AUC 0.65 (95% CI 0.58, 0.72), sensitivity 71%, specificity 59%, LR+ 1.72, LR- 0.50, P < 0.05]. In contrast digital assessment by the Bishop score neither predicted vaginal delivery nor the duration of labour. However, Bishop score predicted time to onset of labour > 12 hours and induction-delivery interval < 24 hrs [optimal cut-off ≤ 4, AUC 0.8 (95% CI 0.75, 0.86), sensitivity 77%, specificity 76%, LR+ 3.3, LR- 0.3, P < 0.05] whilst CR did not.
Cervical resistivity appears predictive of labour duration and delivery mode following induced labour. However the low predictive values obtained suggest that its current design proffers no immediate clinical utility.
Approximately 1 in 5 pregnancies requires induction of labour, a process which carries a high caesarean section rate compared to spontaneous labour . Unwanted outcomes such as caesarean section, prolonged labour, postpartum haemorrhage and traumatic birth often result when labour is induced despite an unfavourable cervix . Pre-labour cervical preparation for birth involves a remodelling process called 'ripening'. Quantifying ripening may enable prediction of the spontaneous initiation of labour, and may inform the timing of induction for clinical indications.
Subjective digital examination of the cervix, often summarized in a composite score such as that described by Bishop  is the traditional method of assessing whether the cervix is favourable for induction or not. The clinical parameters that constitute the Bishop Score (BS) include cervical consistency, length, position and dilatation, as well as the station of the fetal presenting part. Although some studies report that the Bishop score correlates with the ease of artificial initiation of labour, the interval to the onset of labour, and the duration of labour  several others have found no correlation suggesting limited clinical utility [5, 6]. The predictive value of sonographic assessment of cervical length for the onset of labour and labour inducibility is also conflicting [6–10].
Electrical impedance Spectroscopy (EIS) has been employed to study the cervix in vitro [11, 12] and in vivo in non-pregnant [13, 14] and pregnant women [15–17]. We reported normal values of cervical resistivity (CR) during pregnancy  and have observed that CR values are affected by the diameter of the measuring impedance probe: the fraction of injected electrical current that penetrates cervical stroma increases with increasing diameter . Previous studies have been conflicting regarding whether cervical resistivity is able to discriminate between the ripe and unripe cervix prior to induction of labour [15–17]. These studies were undertaken at varied electrical frequencies and employed measurement probes of different dimensions, making comparisons of studies difficult. We hypothesised that optimising cervical probe dimensions and standardising electrical frequencies at which studies are undertaken may facilitate the identification of any clinical utility for this technology.
Since cervical stromal changes associated with ripening may be paralleled by changes in the cervical epithelium  we also hypothesized that smaller measurement probes may capture cervical epithelial resistivity changes with greater sensitivity whilst larger diameter probes would derive cervical stromal resistivity more accurately. We therefore sought to; a) determine cervical tissue resistivity values obtained by four cervical probes with tips of diameter 3 mm, 6 mm, 9 mm and 12 mm; b) assess whether CR obtained with any of the probes correlates with any of the clinical parameters that constitute the Bishop score; c) compare the predictive value of CR to BS for the time to onset of labour, labour duration, ease of labour initiation, successful vaginal delivery, and caesarean section for delayed progress in labour.
We recruited 205 women admitted for elective induction of labour at term (> 37 completed weeks gestation). Written informed consent was obtained from each participant. The study was approved by the South Sheffield Research Ethics Committee (Reference number: 06/Q2305/141). Participants were excluded if they had any of the following: abnormal cervical smear in the previous 3 years, previous caesarean section, previous cervical surgery, multiple pregnancy, ruptured fetal membranes, reproductive birth defects, or cervical dilatation > 3 cm. Only women who had had a normal cervical smear in the 3 years prior to the study were recruited.
Characteristics of 4 tetrapolar probes used in the study.
Pitch circle diameter
(between centres, mm)
The clinical outcomes against which cervical resistivity and Bishop scores were correlated included the following: time to onset of established labour (cervix > 3 cm dilated and at least two uterine contractions every 10 minutes), the duration of labour, the requirement for, and the maximum dose of, syntocinon required for augmentation of labour and successful vaginal delivery. Women whose sole indication for abdominal delivery was suspected fetal compromise were excluded from analysis.
Data were tested for normality of distribution using the Kolmogorov-Smirnov test. Parametric and nonparametric tests as appropriate were used to make comparisons of data obtained with different probes. Correlations between Bishop score and CR values for individual probes on the one hand and the labour characteristics and outcomes on the other were assessed by logistic and stepwise multilinear regression as appropriate. Accuracy of prediction of categorical clinical outcomes by CR and Bishop score was determined from the area under the Receiver Operator Characteristic (ROC) curves, summarized as optimal predictive cut-offs, and positive (LR+) and negative (LR-) likelihood ratios.
Demographic data of participants in study (N = 205)
AGE (MEAN ± SD) years
29.0 ± 6.5
BMI (MEAN ± SD) kg/m-2
27.0 ± 6.2
PARITY n (%)
- Term deliveries only
- Pre-term deliveries only
- Term + preterm deliveries
ETHNICITY n (%)
- South Asian (Indian/Pakistani)
- Mixed Race
SMOKING n (%)
REASON FOR INDUCTION n (%)
- Hypertensive disorders
- Diabetes Mellitus
- Disorders of growth (LGA/SGA*)
- Obstetric Cholestasis
- Disorders of liquor volume
- Reduced fetal movements
- Ante-partum haemorrhage
- Anti-phospholipid syndrome
Clinical/outcomes data of participants in study (n = 205)
Gestation at induction in days, median (range)
280 (259 - 296)
Bishop score, median (range)
5.(1 -- 10)
Method of induction of labour, n(%)
- Prostaglandin only
- Prostaglandin + amniotomy (ARM)
- ARM only
Syntocinon augmentation, n(%)
- Dose of Syntocinon, mu/min, mean (SD)
Duration of labour, mins, mean (SD)
Labour duration <24 hrs, n(%)
Labour duration ≥24 hrs, n(%)
Mode of delivery, n(%)
- Spontaneous vaginal delivery
- Instrumental vaginal delivery
- Caesarean section
Indication for caesarean n(%)
- Delayed progress
- Unsuccessful induction of labour
- Suspected fetal compromise
There was no correlation between CR obtained with any of the four probes and the Bishop score. Unlike the other three probe diameters, lower resistivity values were obtained with the 12 mm probe tip in the range of 9.8 - 78.1 kHz in women who were parous compared to nulliparous women (mean CR at 19.5 kHz 2.15 vs. 2.38 Ω.m, P < 0.01).
Prediction of induced labour by CR vs. Bishop scores
Cervical resistivity obtained with the 3, 6 and 9 mm probes did not correlate with any labour characteristics whilst data obtained with the 12 mm probe did. The latter are compared to the Bishop score below for prediction of time to onset of labour, labour duration, induction-delivery interval and successful vaginal delivery.
Prediction of time to onset of labour (labour latent)
Prediction of time to onset of labour > 12 hours by the Bishop Score vs. cervical resistivity (CR) measured with a 12 mm probe (data shown for 4 frequencies 9.8-78.1 kHz).
AUC (95% CI)
Optimal cut-off value
Sensitivity (95% CI)
Specificity (95% CI)
87.9 (77.5 - 94.6)
67.7 (58.8 - 75.9)
Frequency of CR
95.2 (86.7 - 99.0)
18.03 (11.7 - 26.0)
46.03 (33.4 - 59.1)
64.75 (55.6 - 73.2)
49.21 (36.4 - 62.1)
61.5 (52.2 - 70.1)
26.98 (16.6 - 39.7)
81.97 (74.0 - 88.3)
Prediction of duration of labour
Prediction of labour duration ≥ 24 hrs -- Bishop score vs. cervical resistivity with 12 mm probe (data shown for 4 frequencies 9.8-78.1 kHz).
AUC (95% CI)
Optimal cut-off value
Sensitivity % (95% CI)
Specificity % (95% CI)
0.54 (0.47 - 0.61)
34.6 (17 - 56)
86.0 (80 - 91)
Frequency of CR
92 (70.8 - 98.6)
29 (24.6 - 38.9)
58.3 (37.0 -- 78.0)
66 (58.0 -- 73.0)
0.66 (0.59 --0.73)*
75.00 (49.8 - 89.2)
56.00 (48.3 - 63.5)
70.8 (45.1 - 86.1)
58.9(52.3 - 67.3)
Prediction of vaginal delivery
Prediction of vaginal delivery -- Bishop score vs. cervical resistivity with 12 mm probe (data shown for 4 frequencies 9.8-78.1 kHz).
AUC (95% CI)
Optimal cut-off value
Sensitivity % (95% CI)
Specificity % (95% CI)
0.57 (0.50- 0.64)
30.30 (15.6 - 48.7)
86.0 (79.8 - 90.8)
Frequency of CR
93.55 (78.5 - 99.0)
30.36 (23.5 - 37.9)
87.10 (70.1 - 96.3)
35.12 (27.9 - 42.8)
70.97 (52.0 - 85.7)
53.57 (45.7 - 61.3)
70.97 (52.0 - 85.7)
56.55 (48.7 - 64.2)
Prediction of delivery within 24 hrs of assessment
Syntocinon use for augmentation of labour
Cervical resistivity at 78.1 kHz with the 12 mm probe was significantly higher in women who required syntocinon augmentation of labour (n = 147) compared to those who did not (n = 58) (mean ± SE: 2.30 ± 0.04 vs. 2.18 ± 0.05 Ω.m, P < 0.05, respectively). In contrast the Bishop score did not differ (5.42 ± 0.15 vs. 5.20 ± .0.24, P = 0.44 respectively). Neither the Bishop score nor CR predicted the need for syntocinon acceleration during induced labour.
This is the first study comparing digital assessment of cervical ripening to cervical electrical resistivity measured by a bio-impedance technique for the prediction of the outcome of induced labour. We have demonstrated that CR obtained with largest probe size studied - the 12 mm diameter probe - correlated with parity, the duration of labour and successful vaginal delivery in the frequency range 9.8-78.1 kHz. This correlation suggests that impedance spectroscopy of the cervix reflects electrical properties of the tissue which may be relevant to the physiology of normal and dysfunctional labour. However the predictive likelihood ratios attained were low, precluding its immediate application in clinical settings to predict the outcome of induced labour. The Bishop score prior to induction did not correlate with the duration of labour and was not predictive of vaginal delivery. However it was highly predictive of time to onset of labour greater than 12 hours and of delivery within 24 hours of the assessment.
Consistent with our original hypothesis based on computational modelling [12, 18] only data obtained with the widest diameter probe (12 mm) correlated with parity and duration of labour, and modestly predicted labour duration > 24 hours and vaginal delivery. The greater inter-electrode distance on the large diameter probe ensured that a higher fraction of injected electrical current penetrated cervical stroma to capture its properties via the sensing electrodes, the stroma of the cervix being the principal site of the remodelling process prior to birth [19–24]. Cervical resistivity may better assess cervical stromal "compliance" than digital assessment, and high cervical stromal resistivity may reflect higher resistance to stretch, which may contribute to labour dystocia, fetal distress and caesarean delivery. Consistent with this thesis, previous studies have shown a positive correlation between cervical collagen concentration and the duration of labour, [24, 25]. A limitation of our study was that the numbers of women who required caesarean section for failure to progress and failed induction were too small for us to determine any correlation between CR and caesarean delivery solely for these indications.
We observed a progressive fall in mean cervical resistivity at all frequencies as probe dimensions increased, consistent with our previous observations . The smaller probes are more sensitive to superficial epithelial tissue changes and demonstrate higher resistivity values at low frequencies but do not interrogate deeper sub-epithelial tissues well. The failure of the 3, 6, and 9 mm diameter probes to correlate with labour outcomes whilst the largest probe did, suggests that stromal rather than epithelial tissue elements influence the remodelling processes associated with birth.
The poor predictive value of the Bishop score for successful vaginal delivery in our series agrees with several other reports [5, 6, 26] but conflicts with others [10, 27]. All studies demonstrate that Bishop scores yield low positive predictive likelihood ratios for abdominal delivery, highlighting the limited utility of this assessment. We have observed that the Bishop score is highly predictive of time to onset of established uterine contractions and, consequently, of the induction-delivery interval whilst CR is not. Additionally we have found no correlation between CR values and the Bishop score in contrast to a previous study at a single frequency that suggested an inverse correlation . Taken together, our observations suggest that the Bishop score reflects cervical readiness for labour while CR reflects potential cervical compliance during labour.
The limited predictive value of CR for vaginal delivery suggests that substantial device enhancements would be required before this technique may find clinical utility. Factors which may increase the variability of current impedance measurement probes include the degree of probe pressure on the cervix  and the presence of cervical mucus . In one study, increasing the pressure of the probe on cervical tissue increased tissue resistivity by up to 80%, potentially higher than any pathological or physiological changes of interest . With regard to our current studies, if the CR changes associated with pre-labour remodelling are of a lesser magnitude than may be caused by probe pressure on the cervix, any correlation between CR and labour duration or outcomes may be lost or attenuated. The effect of conductive fluid such as surface mucus on trans-epithelial impedance measured using a tetrapolar probe may be counteracted by the use of a guard electrode . Epithelial tissue boundaries such as the squamo-columnar junction of the cervix modify and confound the impedivity spectra obtained across them . Larger probes are more likely to measure across the squamo-columnar junction given the limited surface area of the ectocervix. We may therefore have underestimated the performance of the 12 mm diameter probe.
Cervical EIS appears a potentially useful diagnostic tool for assessing pre-labour cervical remodelling and for predicting some of the outcomes of induced labour. Although we demonstrated small correlations between cervical resistivity and duration of labour and mode of delivery for the large 12 mm diameter measurement probe, the device performance did not achieve sufficient accuracy to enable employing this tool for clinical purposes at present. Further improvements in the design and application of this technique may enhance its potential application to clinical use.
We would like to thank all the women who agreed to participate in the study. We are grateful to Dr. Jamie Healey and Mr. Michael Anthony from the Department of Medical Physics and Engineering for their contributions to the development and maintenance of the impedance equipment. This work is generously supported by research grants from the Sheffield Teaching Hospitals Charitable Trust and Genisent Medical Technologies USA.
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- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2393/9/40/prepub