Table 1 Sequences of the primers used in QRT-PCR
Target gene | Primer | Nucleotide sequence |
|---|---|---|
AGT | F | 5’-CAGGTCGCAATGATCGCCA-3’ |
R | 5’-GTGTCCATCTAGTCGGGAGGT-3’ | |
ACE1 | F | 5’-TGCCAAGCTCAATGGCTACA-3’ |
R | 5’-GGTCTTGCTCCAGGTTGTCA-3’ | |
AT1R | F R | 5’-TGCCATGCCCATAACCATCTG-3’ 5’-CGTGCTCATTTTCGTAGACAGG-3’ |
AT2R | F R | 5’-ACTTTATGAATGGCATCGAGCTT-3’ 5’-GGGGATAAGAACCTAAACAGCAC-3’ |
β-actin | F R | 5’-GTCGTACCACAGGCATTGTGATGG-3’ 5’-GCAATGCCTGGGTACATGGTGG-3’ |