Fig. 5

Effect of p16 deletion on ovarian stromal abnormalities in mice treated with BUL + CTX. Three months after administering AAs, the ovaries were removed and sectioned. Representative photos of ovarian sections stained with (A) picrosirius red, 6X, and (B) The percentage of PRS positive area. n = 3 mice in each group. Representative photos of ovarian sections stained with (C) α-SMA antibody, 200X, and (D) the percentage of vascularized area visualized by α-SMA, n = 3 mice in each group. Representative photos of ovarian sections stained with (E) CD31 antibody, 200X, and (F) the percentage of vascularized area visualized by CD31, n = 3 mice in each group. Compared with WT mice: ^*P < 0.05; ^**P < 0.01. Compared with p16 KO mice: ^#P < 0.05.WT: WT mice; WT + M: WT mice treated with BUL + CTX; KO mice: untreated p16 KO mice; KO + M: p16 KO mice treated with BUL + CTX.