Genes | Primer sequences 5′ to 3′ | PCR product size (bp) | Initial denaturation | Denaturation | Annealing | Extension | Final Extension | References |
---|---|---|---|---|---|---|---|---|
ermB | TGGTTTTTGAAAGCCATGCGTCTGA | 211 | 95 °C for 5 min | 95 °C for 30 s | 60 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | [16] |
GGAACATCTGTGGTATGGCGGGTAAGTT | ||||||||
MefA/E | AGTATCATTAATCACTAGTGC | 346 | 95 °C for 5 min | 95 °C for 30 s | 50 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | [16] |
TTCTTCTGGTACTAAAAGTGG | ||||||||
ermTR | GAAGTTTAGCTTTCCTAA | 395 | 95 °C for 5 min | 95 °C for 30 s | 50 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | [16] |
GCTTCAGCACCTGTCTTAATTGAT | ||||||||
tetO | AACTTAGGCATTCTGGCTCAC | 515 | 95 °C for 5 min | 95 °C for 30 s | 55 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | [16] |
TCCCACTGTTCCATATCGTCA | ||||||||
tetM | AGTTTTAGCTCATGTTGATG | 1826 | 95 °C for 5 min | 95 °C for 30 s | 51 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | [16] |
TCCGACTATTTGGACGACGG | ||||||||
aphA-3 | AGCTGCCTGTTCCAAAGGTCCTGC | 305 | 95 °C for 5 min | 95 °C for 30 s | 51 °C for 30 s | 72 °C for 35 s | 72 °C for 10 min | [16] |
CAGCTCGCGCGGATCTTTAAATGG |