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Table 1 The primers used for amplification of the antibiotic resistance genes and the PCR conditions

From: Antibiotic resistance genes and molecular typing of Streptococcus agalactiae isolated from pregnant women

Genes

Primer sequences 5′ to 3′

PCR product size (bp)

Initial denaturation

Denaturation

Annealing

Extension

Final Extension

References

ermB

TGGTTTTTGAAAGCCATGCGTCTGA

211

95 °C for 5 min

95 °C for 30 s

60 °C for 30 s

72 °C for 35 s

72 °C for 10 min

[16]

GGAACATCTGTGGTATGGCGGGTAAGTT

MefA/E

AGTATCATTAATCACTAGTGC

346

95 °C for 5 min

95 °C for 30 s

50 °C for 30 s

72 °C for 35 s

72 °C for 10 min

[16]

TTCTTCTGGTACTAAAAGTGG

ermTR

GAAGTTTAGCTTTCCTAA

395

95 °C for 5 min

95 °C for 30 s

50 °C for 30 s

72 °C for 35 s

72 °C for 10 min

[16]

GCTTCAGCACCTGTCTTAATTGAT

tetO

AACTTAGGCATTCTGGCTCAC

515

95 °C for 5 min

95 °C for 30 s

55 °C for 30 s

72 °C for 35 s

72 °C for 10 min

[16]

TCCCACTGTTCCATATCGTCA

tetM

AGTTTTAGCTCATGTTGATG

1826

95 °C for 5 min

95 °C for 30 s

51 °C for 30 s

72 °C for 35 s

72 °C for 10 min

[16]

TCCGACTATTTGGACGACGG

aphA-3

AGCTGCCTGTTCCAAAGGTCCTGC

305

95 °C for 5 min

95 °C for 30 s

51 °C for 30 s

72 °C for 35 s

72 °C for 10 min

[16]

CAGCTCGCGCGGATCTTTAAATGG