Fig. 4

A-D The expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 in chorionic villi cultured with a high concentration of IL-6 (2–100 ng/ml) for 24 h and the correlations of these expression levels, as measured by Western blotting analysis. The expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 (A), the correlation of the IL-6 concentration added to chorionic villi cultures with the expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 (B), the correlation of IDO1 expression with the expression of SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 (C) and the correlation of SOCS3 expression with the expression of SHP-1 and SHP-2 (D) in chorionic villi cultured with high concentrations of IL-6 (2–100 ng/ml) for 24 h were evaluated and analysed. The data are presented as the mean ± SE of three similar experiments. B The correlation coefficients (r) between the IL-6 concentration added to chorionic villi cultures and the expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.9841, 0.9259, 0.9554, 0.9689, 0.9826, and − 0.8904, respectively; the P values for the correlations between IL-6 and IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.0464, 0.0387, 0.0225, 0.0157, 0.0087 and 0.008, respectively. C The correlation coefficients (r) of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.9776, 0.9981, 0.9928, 0.9672, and − 0.9458, respectively; the P values for correlation of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.0112, 0.0041, 0.0036, 0.0165, and 0.0275, respectively. D The correlation of the protein expression levels of SOCS3 and SHP-1/2 was − 0.9670/− 0.9545, respectively; the P value for correlation of the protein expression levels of SOCS3 and SHP-1/2 was 0.0167/0.0230, respectively. E-H The expression and correlation of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 in decidua cultured with high concentrations of IL-6 (2–100 ng/ml) for 24 h, as measured by Western blotting analysis. The expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 (E), the correlation of IL-6 concentration added to the decidua cultures with the expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 (F), the correlation of IDO1 expression with the expression of SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 (G), and the correlation of SOCS3 expression with the expression of SHP-1 and SHP-2 (H) in decidua cultured with high-concentrations of IL-6 (2–100 ng/ml) for 24 h were evaluated and analysed. The data are presented as the mean ± SE of three similar experiments. F The correlation coefficients (r) between the IL-6 concentration added to the decidua cultures and the expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.8031, 0.9572, 0.9330, 0.9858, 0.7722 and − 0.8904, respectively; the P values for the correlations between IL-6 expression and IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 expression were 0.0424, 0.0408, 0.0341, 0.0071, 0.0462 and 0.0462, respectively. G The correlation coefficients (r) of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.9272, 0.9556, 0.8372, 0.9956 and − 0.9717, respectively; the P values for the correlation of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.0371, 0.0224, 0.0420, 0.0022 and 0.0142, respectively. H The correlation of the protein expression levels of SOCS3 and SHP-1/2 was − 0.9575/− 0.993, respectively; the P value for correlation of the protein expression levels of SOCS3 and SHP-1/2 was 0.0215/0.0032, respectively. The greyscale ratios of IDO1, SOCS3, SHP-1, SHP-2, STAT3 and pSTAT3 and the expression levels of IDO1, SOCS3, SHP-1/2, STAT3 and pSTAT3 were assessed based on the ratios of the greyscale values of the IL-6 and IDO1, SOCS3, SHP-1, SHP-2, STAT3 and pSTAT3 bands to that of the GAPDH band. IDO1, indoleamine 2,3-dioxygenase; SOCS3, suppressors of cytokine signalling 3; STAT3, signal transducer and activator of transcription 3; pSTAT3, phosphorylated STAT3; SHP-1/2, protein tyrosine phosphatase-1/2; IL-6, interleukin-6; GAPDH, glyceraldehyde 3-phosphate dehydrogenase