Fig. 3
A-D The expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 in chorionic villi cultured with a low concentration of IL-6 (0–2 ng/ml) for 24 h and the correlation of these expression levels, as measured by Western blotting analysis. The expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 (A), the correlation of the IL-6 concentration added to chorionic villi cultures with the expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 (B), the correlation of IDO1 expression with the expression of SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 (C), and the correlation of SOCS3 expression with the expression of SHP-1 and SHP-2 (D) in chorionic villi cultured with low concentrations of IL-6 (0–2 ng/ml) for 24 h were evaluated and analysed. The data are presented as the mean ± SE of three similar experiments. B The correlation coefficients (r) between the IL-6 concentration added to chorionic villi cultures and the expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.992, 0.988, 0.951, 0.995, 0.962, and 0.9458, respectively; the P values for the correlations of the levels of IL-6 and IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.0175, 0.0427, 0.0445, 0.0426, 0.0412 and 0.0447, respectively. C The correlation coefficients (r) of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.9824, 0.9906, 0.9911, 0.9511 and 0.9327, respectively; the P value for correlation of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.037, 0.0427, 0.0401, 0.0477, and 0.0413, respectively. D The correlation of the protein expression levels of SOCS3 and SHP-1/2 was 0.9883 and 0.9730, respectively; the P value for correlation of the protein expression levels of SOCS3 and SHP-1/2 was 0.0421 and 0.0352, respectively. E-H The expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 in decidua cultured with a low concentration of IL-6 (0–2 ng/ml) for 24 h and the correlations of these expression levels, as measured by Western blotting analysis. The expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 (E), the correlation of the IL-6 concentration added to the decidua cultures with the expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 (F), the correlation of IDO1 expression with the expression of SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 (G), and the correlation of SOCS3 expression with the expression of SHP-1 and SHP-2 (H) in decidua cultured with low concentrations of IL-6 (0–2 ng/ml) for 24 h were evaluated and analysed. The data are presented as the mean ± SE of three similar experiments. F The correlation coefficients (r) between the IL-6 concentration added to the decidua cultures and the expression of IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 were 0.950, 0.951, 0.980, 0.950, 0.9345, and 0.991, respectively; the P values for the correlations of IL-6 expression and IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 expression were 0.0452, 0.0446, 0.0285, 0.0450, 0.0412, and 0.0193, respectively. G The correlation coefficients (r) of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.976, 0.9866, 0.9967, 0.987 and 0.989, respectively; the P value for correlation of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.0007, 0.0437, 0.0124, 0.0002, and 0.0345, respectively. H The correlation of the protein expression levels of SOCS3 and SHP-1/2 was 0.973/9630, respectively; the P value for the correlation of the protein expression levels of SOCS3 and SHP-1/2 was 0.0331/0.0452. The greyscale ratios of IDO1, SOCS3, SHP-1, SHP-2, STAT3 and pSTAT3 and the expression levels of IDO1, SOCS3, SHP-1/2, STAT3 and pSTAT3 were assessed based on the ratios of the greyscale values of the IL-6 and IDO1, SOCS3, SHP-1, SHP-2, STAT3 and pSTAT3 bands to that of the GAPDH band. IDO1, indoleamine 2,3-dioxygenase; SOCS3, suppressors of cytokine signalling 3; STAT3, signal transducer and activator of transcription 3; pSTAT3, phosphorylated STAT3; SHP-1/2, protein tyrosine phosphatase-1/2; IL-6, interleukin-6; GAPDH, glyceraldehyde 3-phosphate dehydrogenase