Fig. 1
The expression of IL-6, IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 and the correlation of these expression levels. A-D The expression of IL-6, IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 and the correlation of these expression levels in chorionic villi. A. The expression of IL-6, IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 in chorionic villi, as measured by Western blotting analysis. B, C and D The correlation of protein expression levels of IL-6, IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 in chorionic villi. Data represent a portion of each sample from thirty-eight healthy pregnant women. Labels 1–8 represent different samples from healthy pregnant women. B The correlation coefficients (r) of the protein expression levels of IL-6 and IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.735, 0.72, 0.77, 0.8047, 0.767, and − 0.844, respectively; the P values for the correlation of the protein expression levels between IL-6 and IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 were 0.0377, 0.0400, 0.0241, 0.0160, 0.0262, and 0.0083, respectively. C The correlation coefficients (r) of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.719, 0.7746, 0.78, 0.7147 and − 0.8923, respectively; the P values for correlation of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 were 0.0443, 0.0240, 0.0198, 0.0463, and 0.0029, respectively. D The correlation of the protein expression levels of SOCS3 and SHP-1/2 was − 0.824/− 0.8773, respectively; the P value for the correlation of the protein expression levels of SOCS3 and SHP-1/2 was 0.0117/0.0042, respectively. E-H The expression of IL-6, IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 and the correlation of these expression levels in decidua. E The expression of IL-6, IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 in the decidua, as measured by Western blotting analysis. F, G and H The correlation of the protein expression levels of IL-6, IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 in decidua. The data represent a portion of each sample from thirty-eight healthy pregnant women. Labels 1–8 represent different samples from healthy pregnant women. F The correlation coefficients (r) of the protein expression levels of IL-6 and IDO1, SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 were 0.8914, 0.8947, 0.8846, 0.8694, 0.8949, and − 0.7950, respectively; the P values for the correlation of the protein expression levels of IL-6 and IDO1, SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.0029, 0.0027, 0.0035, 0.0050, 0.0027 and 0.0183, respectively. G The correlation coefficients (r) of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3 and SOCS3 were 0.834, 0.9890, 0.7359, 0.8527, and − 0.7896, respectively; the P values for correlation of the protein expression levels of IDO1 and SHP-1, SHP-2, STAT3, pSTAT3, and SOCS3 were 0.010, < 0.0001, 0.0374, 0.0071, and 0.0198, respectively. H The correlation of the protein expression levels of SOCS3 and SHP-1/2 was − 0.712/− 0.7665, respectively; the P value for correlation of the protein expression levels of SOCS3 and SHP-1/2 was 0.0451/0.0265, respectively. The greyscale ratios of IL-6 and IDO1, SOCS3, SHP-1, SHP-2, STAT3 and pSTAT3 and the expression levels of IL-6 and IDO1, SOCS3, SHP-1/2, STAT3 and pSTAT3 were assessed based on the ratios of the greyscale value of the IL-6 and IDO1, SOCS3, SHP-1, SHP-2, STAT3 and pSTAT3 bands to that of the GAPDH band. IDO1, indoleamine 2,3-dioxygenase; SOCS3, suppressors of cytokine signalling 3; STAT3, signal transducer and activator of transcription 3; pSTAT3, phosphorylated STAT3; SHP-1/2, protein tyrosine phosphatase-1/2; IL-6, interleukin-6. GAPDH, glyceraldehyde 3-phosphate dehydrogenase