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Fig. 2 | BMC Pregnancy and Childbirth

Fig. 2

From: Triclosan activates c-Jun/miR-218-1-3p/SLC35C1 signaling to regulate cell viability, migration, invasion and inflammatory response of trophoblast cells in vitro

Fig. 2

TCS induces c-Jun to up-regulate miR-218-1-3p at transcriptional level. A Luciferase reporter assay examined the luciferase activity of MIR218–1 promoter after TCS treatment. B The protein level of c-Jun was analyzed by western blot in trophoblasts under different concentrations of TCS. C ChIP assay verified the interaction between c-Jun and MIR218–1 promoter. D Western blot examined the efficiency of pcDNA3.1-JUN. E The luciferase activity of MIR218–1 promoter was tested after JUN was overexpressed. F MiR-218-1-3p expression was examined after transfection of pcDNA3.1-JUN. G ChIP assay detected the abundance of MIR218–1 promoter in c-Jun antibody under the treatment of TCS. H Western blot analyzed the protein levels of c-Jun in the Control group, TCS group and TCS + sh-JUN group. I RT-qPCR analyzed the expression of miR-218-1-3p in indicated groups. **P < 0.01

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BMC Pregnancy and Childbirth

ISSN: 1471-2393

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