Skip to main content

Table 2 Sequence of primers used for Q-RT-PCR analysis

From: Expression of mRNAs for pro-and anti-apoptotic factors in granulosa cells and follicular fluid of women undergoing in vitro fertilization. A pilot study

Gene name

Forward primer sequences (5′-3′)

Reverse primer sequence (5′-3′)

BCL2

GGCCAGGGTCAGAGTTA

CCTCTCTTGCGGAGTATTTG

LHR

GAGATGCACTGTCCAATCC

CCGAATCGAGAGCTGTAATG

FSHR

GGAAGAATCAGGTGGATGG

GGGAGGTCAGAAGGAATCT

AMH

CTTCCTGGAGACGCTCA

GGTCCGACAGGTTGACTA

AMHR2

GGGCTTTGGGCATTACTT

TGTAGAGGCAGCGGATAG

CYP19A1

CAGTGCCTGCAACTACTAC

CCCGAATCGAGAGCTGTAATG

CASP3

CTGAGCCATGGTGAAGAAG

CGGCAGGCCTGAATAATG

CASP8

CCAGTGGGCAAGAGAATTAG

CAAGTGACCAACTCAAGGG

BAX

GAGCTGCAGAGGATGATTG

GCCTTGAGCACCAGTTT

HPRT1

TGCTTCTCCTCAGCTTCA

CTCAGGAGGAGGADGCC

  1. Abbreviations: BCL2 BCL2 apoptosis regulator, LHR Luteinizing hormone receptor, FSHR Follicle stimulating hormone receptor, AMH Anti-Müllerian hormone, AMHR2 Anti-Müllerian hormone receptor 2, CYP19A1 Cytochrome P450 family 19 subfamily A member 1, CASP3 Caspase 3, CASP8 Caspase 8, BAX BCL2 associated X apoptosis regulator, HPRT1 Hypoxanthine phosphoribosyl transferase 1 (housekeeping gene)
Back to article page

BMC Pregnancy and Childbirth

ISSN: 1471-2393

Contact us