Fig. 7

CXCL12 stimulated the activation of Akt, PI3K and ERK in EECs. Human first-trimester endometrial epithelial cells (EECs) were serum starved for 12 h and then stimulated with CXCL12 (100 ng/ml). The phosphorylation of protein kinase B (AKT), extracellular regulated protein kinases (ERK) and phosphatidylinositol 3-kinase (PI3K) were evaluated with in-cell western blot analysis. CXCL12 (at 100 ng/ml) led to time-dependent increase in phosphorylation of AKT, PI3K and ERK1/2 in EECs. Phosphorylated proteins were stained in green and total proteins stained in red. The phosphorylated to total protein ratio was normalized to 1 in the untreated control (n = 3)