Fig. 6

Immunofluorescence staining of term placenta with various antibodies. Counter-staining of nuclei with Dapi (blue). a-c) VEGFR-3 (green) is expressed in a few intra- and extra-vascular leukocytes (arrowheads in A,B). Bar = 35 μm. C) Negative control without primary antibody. d-f) CD34 (red) and LYVE1 (green) double-staining. CD34 is expressed in endothelial cells, LYVE1 in scattered stromal cells. E) Negative control without primary antibody. Bar = 120 μm in D, and 30 μm in F. g-i) CCBE1 (red) and D2–40/PDPN (green) double-staining. CCBE1 is expressed in endothelial cells, PDPN in numerous stromal cells. H) Negative control without primary antibody. Bar = 120 μm in H, and 30 μm in I. k-m) Vimentin is expressed in endothelial cells and stromal cells of terminal villi (K) and stem villi (L). M) Negative control without primary antibody. Bar = 30 μm in K, and 120 μm in M