Fig. 3

Amplification curves of measured parameters. Positive, negative and no-template controls were included in all reactions. a Standard curve (2E04–2 GE/μL) constructed to determine the anticipated reportable range. b Amplification of curves (32–4 GE/μL) used to determinate the limit of detection. The pool of samples lacking GBS strains did not produce an amplification signal. c Curves showing the amplification signals from all GBS serotypes tested, used to determine the inclusivity parameter. d Curves for negatives, samples from non-GBS strains, used to determinate the exclusivity parameter. Note. PAC: positive amplification control for GBS