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Table 4 Data from phenotypic characterization and multiplex real-time PCR of Streptococcus agalactiae

From: Development of a melting-curve based multiplex real-time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides

Isolates Susceptibility phenotype Real-time multiplex PCR
  Ea DAb cfb c erm(A)c erm(B)c mef(A/E)c
LMC UEL 5 S S + – – –
LMC UEL 21 S S + – – –
LMC UEL 23 S S + – + –
LMC UEL 27 S S + – – –
LMC UEL 30 S S + – – –
LMC UEL 34 S S + – – –
LMC UEL 43 S S + – – –
LMC UEL 43A R R + + + –
LMC UEL 57 S S + + – +
LMC UEL 60 S S + – – –
LMC UEL 68 S S – – – –
LMC UEL 103 R S + – – +
LMC UEL 28 CN CN + – – –
LMC UEL 63 CN CN + – + +
LMC UEL 95 CN CN + – – +
LMC UEL 99 CN CN + – + +
  1. aE (erythromycin) and bDA (clindamycin) resistance phenotypes were determined by the double-disk diffusion method [34]. (S) Susceptible; (R) Resistant; (CN) Culture-Negative. cTarget genes detected in vaginal-rectal swab specimens by multiplex real-time PCR. (+) Presence; (−) Absence
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BMC Pregnancy and Childbirth

ISSN: 1471-2393

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