Table 1 Panel of microorganisms used to evaluate the multiplex real-time PCR specificity and sensitivity

Streptococcus agalactiae

ATCC 13813

Escherichia coli

ATCC 25922

Streptococcus agalactiae

LMC UEL 15

Escherichia coli

ATCC 35218

Streptococcus agalactiae

LMC UEL 65

Klebsiella pneumoniae

ATCC 700603

Streptococcus agalactiae

LMC UEL 66

Proteus mirabilis

HU-UEL

Streptococcus agalactiae serotype Ia

LMC UEL 43

Providencia stuartii

HU-UEL

Streptococcus agalactiae serotype II

LMC UEL 92

Salmonella sp.

HU-UEL

Streptococcus agalactiae serotype III

LMC UEL 59

Shigella dysenteriae

ATCC 13313

Streptococcus agalactiae serotype V

LMC UEL 73

Enterococcus faecalis

ATCC 29212

Streptococcus agalactiae serotype IX

LMC UEL 11

Enterococcus faecium

ATCC 6569

Streptococcus dysgalactiae subsp. equisimilis group G.

LACEN 6196

Lactobacillus acidophilus

ATCC 4356

Streptococcus dysgalactiae subsp. equisimilis group C

LACEN 53157

Lactobacillus rhamnosus

LBBA-UEL

Streptococcus mitis

ATCC 49456

Lactococcus lactis subsp. lactis

LBBA-UEL 22

Streptococcus mutans

ATCC 25175

Lactococcus lactis subsp. cremoris

LBBA-UEL 22–1

Streptococcus pneumoniae

ATCC 49619

Leuconostoc mesenteroides

LBBA-UEL 704

Streptococcus pyogenes

ATCC 19615

Candida albicans

ATCC 26790

Streptococcus sanguis

ATCC10557

Candida bracarensis

LMC UEL1217

Staphylococcus aureus

ATCC 25923

Candida dubliniensis

LMC UEL 947C

Staphylococcus epidermidis

ATCC 12228

Candida glabrata

LMC UEL 51B

Staphylococcus haemolyticus

ATCC 29668

Candida metapsilosis

LMC UEL 2263

Staphylococcus saprophyticus

HU-UEL

Candida orthopsilosis

LMC UEL 2259

Bacillus subtilis

ATCC 23857

Candida parapsilosis

ATCC 22019

Aeromonas sp.

HU-UEL

Candida tropicalis

ATCC 28707

Pseudomonas aeruginosa

ATCC 27853

Cryptococcus gattii

ATCC 56990

Citrobacter freundii

HU-UEL

Cryptococcus neoformans

ATCC 66031