Table 2 Gradient Elution for Oxytocin
Time (min) | Mobile phase A (% v/v) | Mobile phase B (% v/v) | Comments |
|---|---|---|---|
0–5 | 100 | 0 | Isocratic |
5–20 | 70 | 30 | Linear gradient |
20–50 | 30 | 70 | Linear gradient |
50–51 | 60 | 40 | Return to initial composition |
51–65 | 100 | 0 | Re-equilibration |