Table 2 Gradient Elution for Oxytocin
| Time (min) | Mobile phase A (% v/v) | Mobile phase B (% v/v) | Comments |
|---|---|---|---|
| 0–5 | 100 | 0 | Isocratic |
| 5–20 | 70 | 30 | Linear gradient |
| 20–50 | 30 | 70 | Linear gradient |
| 50–51 | 60 | 40 | Return to initial composition |
| 51–65 | 100 | 0 | Re-equilibration |
| Time (min) | Mobile phase A (% v/v) | Mobile phase B (% v/v) | Comments |
|---|---|---|---|
| 0–5 | 100 | 0 | Isocratic |
| 5–20 | 70 | 30 | Linear gradient |
| 20–50 | 30 | 70 | Linear gradient |
| 50–51 | 60 | 40 | Return to initial composition |
| 51–65 | 100 | 0 | Re-equilibration |