Fig. 1

Leukocyte migration assay performance characteristics. a Migration as a function of amount of chemoattractant placed in the lower chamber. Increasing amounts of homogenate (determined as total protein in μg) were placed in the lower chamber and migrated (attracted) cells counted after performing the assay as in Methods. Different superscripts denote statistical significance, p < 0.05. b. Migration as a function of number of leukocytes placed in the upper chamber. Increasing numbers of cells were placed in the upper chamber and migrated (attracted) cells counted after performing the assay as in Methods. Different superscripts denote statistical significance, p < 0.05