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Table 2 Primers and probes used in this study for qPCR reactions

From: Maternal vitamin D supplementation during pregnancy and lactation to prevent acute respiratory infections in infancy in Dhaka, Bangladesh (MDARI trial): protocol for a prospective cohort study nested within a randomized controlled trial

Primer Name Primer/probe Sequence (5′-3′) Nucleotide Sequence 5′-3′
lytA Forward ACG CAA TCT AGC AGA TGA AGC A
Reverse TCG TGC GTT TTA ATT CCA GCT
Probe (Pb 400) 6FAM-TGC CGA AAA CGC TTG ATA CAG GGA G-MGB
InfA Forward GAC CRA TCC TGT CAC CTC TGA C
Reverse AGG GCA TTY TGG ACA AAK CGT CTA
Probe1 6FAM-TGC AGT CCT CGC TCA CTG GGC ACG-BHQ1
InfB Forward TCC TCA AYT CAC TCT TCG AGC G
Reverse CGG TGC TCT TGA CCA AAT TGG
Probe1 6FAM-CCA ATT CGA GCA GCT GAA ACT GCG GTG-BHQ1
RNP Forward AGA TTT GGA CCT GCG AGC G
Reverse GAG CGG CTG TCT CCA CAA GT
Probe1 6FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ1
RSV Forward GGC AAA TAT GGA AAC ATA CGT GAA
Reverse TCT TTT TCT AGG ACA TTG TAY TGA ACA G
Probe1 6FAM-CTG TGT ATG TGG AGC CTT CGT GAA GCT-BHQ1
HMPV Forward CAA GTG TGA CAT TGC TGA YCT RAA
Reverse ACT GCC GCA CAA CAT TTA GRA A
Probe 1 6FAM-TGG CYG TYA GCT TCA GTC AAT TCA ACA GA-BHQ1
HPIV1 Forward ACA AGT TGT CAA YGT CTT AAT TCR TAT
Reverse TCG GCA CCT AAG TAR TTY TGA GTT
Probe 6FAM-ATA GGC CAA AGA “T”TG TTG TCG AGA CTA TTC CAA
HPIV2 Forward GCA TTT CCA ATC TAC AGG ACT ATG A
Reverse ACC TCC TGG TAT AGC AGT GAC TGA AC
Probe 6FAM-CCA TTT ACC “T”AA GTG ATG GAA TCA ATC GCA AA
HPIV3 Forward TGG YTC AAT CTC AAC AAC AAG ATT TAA G
Reverse TAC CCG AGA AAT ATT ATT TTG CC
Probe1 6FAM-CCC RTC TG“T” TGG ACC AGG GAT ATA CTA CAA A
Adeno Forward GCC CCA GTG GTC TTA CAT GCA CAT C
Reverse GCC ACG GTG GGG TTT CTA AAC TT
Probe 6FAM-TG CAC CAG ACC CGG GCT CAG GTA CTC CGA-BHQ1
  1. Note: Quenched internally at a modified “T” residue with BHQ1 and a terminal phosphate at the 3′-end to prevent probe extension by Taq polymerase