Maternal vitamin D supplementation during pregnancy and lactation to prevent acute respiratory infections in infancy in Dhaka, Bangladesh (MDARI trial): protocol for a prospective cohort study nested within a randomized controlled trial

Table 2 Primers and probes used in this study for qPCR reactions

Primer Name		Primer/probe Sequence (5′-3′) Nucleotide Sequence 5′-3′
lytA	Forward	ACG CAA TCT AGC AGA TGA AGC A
	Reverse	TCG TGC GTT TTA ATT CCA GCT
	Probe (Pb 400)	6FAM-TGC CGA AAA CGC TTG ATA CAG GGA G-MGB
InfA	Forward	GAC CRA TCC TGT CAC CTC TGA C
	Reverse	AGG GCA TTY TGG ACA AAK CGT CTA
	Probe1	6FAM-TGC AGT CCT CGC TCA CTG GGC ACG-BHQ1
InfB	Forward	TCC TCA AYT CAC TCT TCG AGC G
	Reverse	CGG TGC TCT TGA CCA AAT TGG
	Probe1	6FAM-CCA ATT CGA GCA GCT GAA ACT GCG GTG-BHQ1
RNP	Forward	AGA TTT GGA CCT GCG AGC G
	Reverse	GAG CGG CTG TCT CCA CAA GT
	Probe1	6FAM-TTC TGA CCT GAA GGC TCT GCG CG-BHQ1
RSV	Forward	GGC AAA TAT GGA AAC ATA CGT GAA
	Reverse	TCT TTT TCT AGG ACA TTG TAY TGA ACA G
	Probe1	6FAM-CTG TGT ATG TGG AGC CTT CGT GAA GCT-BHQ1
HMPV	Forward	CAA GTG TGA CAT TGC TGA YCT RAA
	Reverse	ACT GCC GCA CAA CAT TTA GRA A
	Probe 1	6FAM-TGG CYG TYA GCT TCA GTC AAT TCA ACA GA-BHQ1
HPIV1	Forward	ACA AGT TGT CAA YGT CTT AAT TCR TAT
	Reverse	TCG GCA CCT AAG TAR TTY TGA GTT
	Probe	6FAM-ATA GGC CAA AGA “T”TG TTG TCG AGA CTA TTC CAA
HPIV2	Forward	GCA TTT CCA ATC TAC AGG ACT ATG A
	Reverse	ACC TCC TGG TAT AGC AGT GAC TGA AC
	Probe	6FAM-CCA TTT ACC “T”AA GTG ATG GAA TCA ATC GCA AA
HPIV3	Forward	TGG YTC AAT CTC AAC AAC AAG ATT TAA G
	Reverse	TAC CCG AGA AAT ATT ATT TTG CC
	Probe1	6FAM-CCC RTC TG“T” TGG ACC AGG GAT ATA CTA CAA A
Adeno	Forward	GCC CCA GTG GTC TTA CAT GCA CAT C
	Reverse	GCC ACG GTG GGG TTT CTA AAC TT
	Probe	6FAM-TG CAC CAG ACC CGG GCT CAG GTA CTC CGA-BHQ1

Note: Quenched internally at a modified “T” residue with BHQ1 and a terminal phosphate at the 3′-end to prevent probe extension by Taq polymerase

ISSN: 1471-2393