Table 3 List of laboratory tests, sample size and proposed biomarkers in the study

Pregnant women between 24–32 weeks

Blood pressure (automated)

5000

OGTT (Fasting plasma glucose, 2 Hour Postprandial plasma glucose)

5000

Glucose

Haemoglobin

5000

Haemoglobin

HbA1c (glycosylated haemoglobin, Type A1C)

500

Glycosylated haemoglobin

One aliquot of fasting sample will be frozen at −80 °C for measurement of serum insulin

1000

Insulin resistance and insulin secretion through homeostasis model assessment (HOMA)

Blood sample to be preserved at−80⁰ C

5000

Micronutrients, DNA and protein markers

Repeat OGTT after 32 weeks (Fasting plasma glucose, 2 Hour Postprandial plasma glucose)

500

To explore those who develop gestational diabetes after 32 weeks

Cord blood

C-peptide

100

Insulin

Fathers

Fasting blood glucose in fathers

500

Glucose

Mothers (During follow up visits)

Random blood glucose

5000

Glucose

At the end of the study (IV year)

Fasting blood glucose and insulin levels in mother and child

5000

Glucose