Table 2 Primers used for quantitating mRNA
mRNA | Primers | Annealing (°C) | Amplicom (bp) |
|---|---|---|---|
GAPDH | F:5′GGGAAACTGTGGCGTGAT3′ | 60 | 299 |
R:5′GAGTGGGTGTCGCTGTTGA3′ | |||
ADAMTS15 | F:5′TGTGAAAGTCTGTGAGGAGGTGT3′ | 60 | 127 |
R:5′CAGGAAGTCGGTGATGATGG3′ | |||
AQPEP | F:5′GGACACGGAATACATGGTGC3′ | 60 | 137 |
R:5′CCTGGTCGGTGTAGACGTTG3′ | |||
EML4 | F:5′TGAAGAGCCATGCAACGAGA3′ | 60 | 95 |
R:5′AGCCAGGGTGTTAGGACGAG3′ | |||
NBPF10 | F:5′ATCAGCTTCGCCCTTTACG3′ | 60 | 82 |
R:5′TGACTCCCATCTGGAACACC3′ | |||
PAM | F:5′CCAGACCCGTAGTTCCTATTGA3′ | 60 | 96 |
R:5′ACATGCAGAAGTATGTATCGGACT3′ | |||
PDE4B | F:5′CAGAAAGCAAGACCAGGAAGC3′ | 60 | 185 |
R:5′GGATAAGTTCCCGAAACTTAGTGC3′ | |||
PPP2R5C | F:5′TTCATCGAGGTCTCGGGTAT3′ | 60 | 97 |
R:5′CTTGAAGTGACAGCAGTGGG3′ | |||
STAM | F:5′TGGCAGTGACACCAAAGATAGAG3′ | 60 | 141 |
R:5′TAGCAAGGGATTAGACAGACGAA3′ | |||
TACC2 | F:5′GACACCTGTGATGAGTCCGTTG3′ | 60 | 149 |
R:5′TTCTTGGCGGGCTTGTTTAG3′ | |||
TATDN1 | F:5′TGAAAACTGAAGCTAATTTGGAA3′ | 60 | 211 |
R:5′ GCAGGGTTCATTTCTGTCTTT3′ |