Figure 3

Localization of CB1, CB2 and FAAH in human placental villi, decidua and cerebellum. CB1 specific staining is shown in normal (A) and preeclamptic (B) placental tissues, images were captured at 200× magnification. Further images demonstrate strong CB1 positivity in preeclamptic placental villi (D) and decidua (E) at 400× magnification. Arrow indicates strong CB1 immunoreactivity in villous capillary endothelial cells. Positive control reaction for CB1 is revealed in human cerebellum (C). CB2 specific staining is shown in normal (F) and preeclamptic (G) placental tissues, and in human cerebellum (H) as a positive control. FAAH specific staining is presented in normal (I) and preeclamptic (J) placental tissues, and in human cerebellum (K) as a positive control. Negative controls are shown in human placental villi and decidua (L), and in human cerebellum (M). Placental tissue samples from normal pregnancy and preeclampsia were captured at 200× magnification, while positive and negative controls were taken at 400× magnification; bar =100 μm.