Parameter | Method of estimation | Equipment |
---|---|---|
Total cholesterol | CHOD-PAP method | COBAS C311 & c501 |
Triglycerides | GPO-PAP method | COBAS C311 & c501 |
Low density lipoprotein-cholesterol | Friedewald formula: | NA |
LDL Cholesterol = (Total Cholesterol) – (HDL- Cholesterol) – (Triglyceride/5) | ||
High density lipoprotein-cholesterol | Phosphotungstic acid precipitation and enzymatic colorimetric method | COBAS C311 & c501 |
C-reactive protein | Sandwich principle Technique. | COBAS C311 & c501 |
Fasting insulin | CLIA technique | COBAS C311 & c501 |
Post prandial insulin | CLIA technique | COBAS C311 & c501 |
Thyroid stimulating hormone | Electro Chemilumiscent technique | COBAS C311 & c501 |
Glycated haemoglobin (HbA1c) | HPLC method | Variant II Turbo-2-BIORAD |