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Table 2 Estimation of lipid profile, HbA1c, insulin, c-reactive protein and thyroid stimulating hormone

From: A cohort study of gestational diabetes mellitus and complimentary qualitative research: background, aims and design

Parameter

Method of estimation

Equipment

Total cholesterol

CHOD-PAP method

COBAS C311 & c501

Triglycerides

GPO-PAP method

COBAS C311 & c501

Low density lipoprotein-cholesterol

Friedewald formula:

NA

LDL Cholesterol = (Total Cholesterol) – (HDL- Cholesterol) – (Triglyceride/5)

High density lipoprotein-cholesterol

Phosphotungstic acid precipitation and enzymatic colorimetric method

COBAS C311 & c501

C-reactive protein

Sandwich principle Technique.

COBAS C311 & c501

Fasting insulin

CLIA technique

COBAS C311 & c501

Post prandial insulin

CLIA technique

COBAS C311 & c501

Thyroid stimulating hormone

Electro Chemilumiscent technique

COBAS C311 & c501

Glycated haemoglobin (HbA1c)

HPLC method

Variant II Turbo-2-BIORAD