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Figure 4 | BMC Pregnancy and Childbirth

Figure 4

From: Adenosine A2Breceptors induce proliferation, invasion and activation of cAMP response element binding protein (CREB) in trophoblast cells

Figure 4

Effect of A 2B adenosine receptor activation on trophoblast integration into endothelial cell monolayers. (A). Representative images of integration of trophoblast cells (red) into confluent endothelial cell (green) monolayers after 48 h of incubation with A2B receptor agonist (NECA, 10 μM) (b, e, h), antagonist (MRS, 1 μM) (c, f, i) and without (a, d, g) at 2% O2, 8% O2 or 21% O2. Trophoblast cells and endothelial cells (co-culture) were incubated at 2% O2, 8% O2 or 21% O2 at 37°C for 48 h in the presence of experimental agents (10 μM NECA or 1 μM MRS 1754). (B). Trophoblast integration as a function of oxygen concentration. Untreated HTR-8/SVneo and HUtMVEC cells were incubated at 2%, 8% or 21% O2. Populated area (%) was analyzed as measure of trophoblast integration (C). Data are presented as mean ± SEM based on 13 independent experiments, *p < 0.05.

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