Figure 2

Effect of NECA and the PKA inhibitor H-89 on CREB phosphorylation in trophoblast cells. Phosphorylation of CREB was analyzed by Western Blot with phospho-CREB antibody and set in relation to total CREB protein after normalization to β-actin levels (A, C). Trophoblast cells were stimulated with an A_2B receptor agonist (NECA, 10 μM) or antagonist (MRS 1754, 1 μM) and H-89 inhibitor (10 μM) for 1 h at 2% O₂, 8% O₂ or 21% O₂. Densitometric quantification of Western Blots (B, D). Data are presented as mean ± SEM based on eight independent experiments, *p < 0.05.