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Figure 1 | BMC Pregnancy and Childbirth

Figure 1

From: Adenosine A2Breceptors induce proliferation, invasion and activation of cAMP response element binding protein (CREB) in trophoblast cells

Figure 1

Effect of different oxygen concentrations on mRNA and protein expression of adenosine receptor A 2B and cyclic cAMP accumulation by NECA in trophoblast cells. Representative Western Blot of adenosine receptor A2B expression in trophoblast cells after 1 h of incubation at 2% O2, 8% O2 or 21% O2 (A). Densitometric quantification of Western Blots. Data are presented as mean ± SEM based on two independent experiments (B). Levels of adenosine receptor A2B mRNA expression 1 h, 4 h and 24 h after incubation at 2% O2, 8% O2 or 21% O2. Data are presented as mean ± SEM based on four independent experiments, *p < 0.05 (C). Accumulation of cAMP induced by the A2B receptor agonist (NECA, 10 μM) or antagonist (MRS 1754, 1 μM) at 2% O2 or 21% O2 (N = 5, *p < 0.05) (D).

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