Table 1 Primer sequences
Name | Primer Sequences (5’ – 3’) | Product Size Conventional Or real-time | Target Gene (GenBank Accession) |
|---|---|---|---|
β2-microglobulin | F: CTTATGCACGCTTAACTATCTTAACAA R: TAGGAGGGCTGGCAACTTAG | 127bp Real-time | B2M NM_004048 |
Calponin | F: GATGGCATCATTCTTTGCGA R: TTGTAGTAGTTGTGTGCGTG F: CTGAGAGAGTGGATCGAGGG R: TGATCTTCTTCACGGAGCCT | 701bp Conventional 127bp Real-time | CNN1 NM_001299 |
Connexin 43 | F: GTGCCTGAACTTGCCTTTTC R: GATGATGTAGGTTCGCAGCA F: GGAGTTCAATCACTTGGCGT R: ACACCTTCCCTCCAGCAGTT | 522bp Conventional 134bp Real-time | GJA1 NM_000165 |
Cyclo-oxygenase-type 2 also known as Prostaglandin GH synthase-2 | F: AGATCATCTCTGCCTGAGTATCTT R: TTCAAATGAGATTGTGGAAAAATTGCT F: GCTGGGCCATGGGGTGGACT R: CCTGCCCCACAGCAAACCGT | 305bp Conventional 200bp Real-time | COX-2 or PTGS-2 NM_000963 |
Glyceraldehyde-phosphatedehydroegnase | F: CCACCCATGGCAAATTCCATGGCA R: TCTAGACGGCAGGTCAGGTCCACC | 598bp Conventional | GAPDH NM_002046 |
Vimentin | F: TCAGAGAGAGGAAGCCGAAA R: GTGAGGGACTGCACCTGTCT F: GGCTCAGATTCAGGAACAGCATG R: CCTGTCTCCGGTACTCAGTGGAC | 380bp Conventional 230bp Real-time | VIM NM_003380 |