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Table 1 Genotyping methods description.

From: No association between polymorphisms/haplotypes of the vascular endothelial growth factor gene and preeclampsia

SNP ID

Primer sequences*

Genotyping method

(restriction endonuclease)

PCR- RFLP products length (bp)

Reference†

rs699947

Fw 5'-GCCTTAGGACACCATACCGATG-3'

Rv 5'-GCTGCCCCAGGGAACAAAGTTG-3'

PCR/RFLP

(Bgl II)

CC: 285

CA: 285,206,79

AA: 206,79

Modified from [3]

rs1570360

Fw 5'-TCCTGCTCCCTCCTCGCCAATG-3'

Rv 5'-GGCGGGGACAGGCGAGCATCAG-3'

PCR/RFLP

(Mnl I)

AA: 206

AG: 206,180,26

GG: 180,26

Modified from [19]

rs2010963

Fw 5'-CCGACGGCTTGGGGAGATTGCTC-3'

Rv 5'-CGGCGGTCACCCCCAAAAGCAG-3'

PCR/RFLP

(BsmF 1)

GG:157, 40

GC: 197,157,40

CC: 197

Modified from [3]

rs25648

----

Real time PCR Taqman assay

----

hCV791476

  1. *Nucleotides in bold letters in primer sequences indicate primer modifications included in this study. †Original references of primer sequences and protocols.
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BMC Pregnancy and Childbirth

ISSN: 1471-2393

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