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Fig. 3 | BMC Pregnancy and Childbirth

Fig. 3

From: Development of a new staining protocol for the Kleihauer–Betke test to facilitate the reading of difficult cases

Fig. 3

Diagnosis performance of the two staining methods. A Results of 30 patients (expected negative), dots having as coordinates the two results of Kleihauer test (with Shepard method and the new method); the blue line represents the trend line of the patients results, and the red line the first diagonal of the graphic. B Diminution of values in proposed method of Kleihauer test represented in percentage reported at the results in Sheppard method (100%) for the patients with abnormal hemoglobin electrophoresis. C Representation of false positive fetal RBCs/10,000 adult RBCs on a logarithmic scale; orange represents the value obtained using Shepard staining, and blue represents the value obtained using our proposed staining method. The red line is the threshold used in our laboratory. D The average of the differences between first and second count for the two methods, with the p result of the Wilcoxon test between the groups

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