Sero-conversion rate of Syphilis and HIV among pregnant women attending antenatal clinic in Tanzania: a need for re-screening at delivery
- John DT Lawi†1,
- Mariam M Mirambo†2Email author,
- Moke Magoma3,
- Martha F Mushi2,
- Hyasinta M Jaka4,
- Balthazary Gumodoka5 and
- Stephen E Mshana2
© Lawi et al.; licensee BioMed Central. 2015
Received: 15 July 2014
Accepted: 12 January 2015
Published: 22 January 2015
Despite the available cost effective antenatal testing and treatment, syphilis and human immunodeficiency virus (HIV) are still among common infections affecting pregnant women especially in developing countries. In Tanzania, pregnant women are tested only once for syphilis and HIV during antenatal clinic (ANC) visits. Therefore, there are missed opportunities for syphilis and HIV screening among those who were not tested during ANC visits and those acquiring infections during the course of pregnancy. This study was designed to determine the syphilis and HIV seroprevalence at delivery and seroconversion rate among pregnant women delivering at Bugando Medical Centre (BMC).
A cross sectional, hospital-based study involving pregnant women attending Bugando Medical Centre (BMC) antenatal clinic was done from January to March 2012. Serum samples were collected and tested for HIV and syphilis using HIV and syphilis rapid tests. Demographic and clinical data were collected using a standardized data collection tool and analysed using STATA version 11.
A total of 331 and 408 women were screened for syphilis and HIV during antenatal respectively. Of 331 women who screened negative for syphilis at ANC, nine (2.7%) were seropositive at delivery while of 391who tested negative for HIV during ANC eight (2%) were found to be positive at delivery. Six (1.8%) and 23 (9%) of women who did not screen for syphilis and HIV at ANC were seropositive for syphilis and HIV at delivery respectively. There was significant difference of seroprevalence for HIV, among women who tested negative at ANC and those who did not test at ANC (2% vs.9%, P,<0.001). The overall prevalence of syphilis and HIV at delivery was 15 (2.3%) and 48 (7.2%) respectively. Syphilis seropositivity at delivery was significantly associated with HIV co-infection (p < 0.001), male partner circumcision (p = 0.011) and alcohol use among women (p < 0.001).
The current protocol of screening for syphilis and HIV only once during pregnancy as practiced in Tanzania may miss women who get re-infected and seroconvert during pregnancy. Re-screening for syphilis and HIV during the course of pregnancy and at delivery is recommended in Tanzania as it can help to identify such women and institute appropriate treatment.
KeywordsSyphilis Treponema pallidum Human immunodeficiency virus Seroprevalence Seroconversion Sexually transmitted infection
Sexually transmitted infections (STIs) are of public health concern in many developing countries including Tanzania. Syphilis and HIV have been of a major concern because of their impact on pregnancy outcome . Syphilis is caused by the bacteria Treponema pallidum, and is categorized as one of the genital ulcerative disease [2,3] while HIV/AIDS is caused by human immunodeficiency virus(HIV). Pregnant women are at high risk of syphilis infection due to pregnancy induced cervical changes, such as hyperaemia, eversion and friability which facilitate easy transmission of syphilis leading to spirochaetaemia .
The World Health Organization (WHO) estimates that about 2 million pregnant women are infected by T.pallidum globally with a 45-70% probability (1.2 million cases) of vertical transmission to neonates annually [5,6]. In developing countries, 3-15% of women of child-bearing age are infected with syphilis [3,7]. Syphilis screening during pregnancy is important strategy to prevent early fetal and neonatal death which is estimated at 620,000 worldwide annually .
As syphilis, HIV infection in pregnancy has become a major concern in many developing countries. Previous reports showed that approximately 1.5 million HIV-positive women become pregnant each year  with mother to child transmission rate estimated at 600 000 annually . Previous studies showed that the incidence of HIV is higher in pregnant women than in non pregnant women . During pregnancy the risk of HIV acquisition increases due to immunological and hormonal changes, resulting in increasing progesterone levels which affects the mucosa of the genital tract [11,12]. HIV infection during pregnancy has been associated with adverse fetal and maternal outcomes .
Early detection and treatment of syphilis and HIV in pregnancy, especially when done before the second trimester, is essential in preventing long term neonatal complications and transmission of disease . Even pregnant women infected with T.pallidum four weeks before delivery can still result in birth of an affected newborn . Moreover, HIV seronegative women who seroconvert in late pregnancy can have maternal fetal transmission . It is recommended that syphilis screening done at the first antenatal clinic (ANC) visit in the first trimester of pregnancy, third trimester for detection of new infections acquired during pregnancy and at delivery for those who missed the opportunity of antenatal screening to allow for an early diagnosis and treatment of infections in women and newborns . Similarly HIV testing in early and late pregnancy or at delivery reduces the risk of mother to child transmission .
However, in developing countries, syphilis testing during antenatal is low, ranging from 30 to 38%. As a result, few women get tested and treated for infections acquired during pregnancy . In Tanzania, women are routinely screened for syphilis and HIV once in the entire pregnancy. The ANC attendance in Tanzania is more than 95% with about 60% hospital delivery. About 42% of women attend ANC at least four times and only 15% of women starts ANC before sixteen weeks of gestation . Thus many women are likely to progress up to delivery without being screened for syphilis and HIV. It is important to establish seroprevalence of these infections among pregnant women at initiation of pre natal care and at delivery in order to evaluate if the single screening method at initiation of prenatal care is effective in detection and control of these infections. In addition, in developing countries including Tanzania, few pregnant women get tested for syphilis during ANC visits,  thus the need for screening at delivery of the non reactive women and those who miss the opportunity of screening during pregnancy. Such data is scarce in Tanzania and this study aimed to provide this information to help health policy makers plan for effective strategies to control syphilis and HIV infections during pregnancy.
This was cross-sectional hospital based study involving women attending Bugando Medical Centre (BMC) labour ward between January and March 2012. BMC is a consultant and teaching hospital for the Lake and Western zones of the United Republic of Tanzania. It is situated along the shores of Lake Victoria in Mwanza City with a bed capacity of 900. It is a referral centre for specialized tertiary care for eight regions: Mwanza, Geita, Mara, Simiyu, Kagera, Shinyanga, Tabora and Kigoma. It serves catchments population of approximately 13 million people (http://www.bugandomedicalcentre.go.tz).
The study included pregnant women who were not tested or tested negative for syphilis and HIV during antenatal care visits who delivered at BMC during the study period. Sample size for this study was calculated by using a formula of Kelsey et al. 1996 . Proportion of exposed with disease (p1) was 3.0%  and proportion of unexposed with disease (p2) was = 8.0% . Using the above proportions, power, confidence interval and ratio, the sample size was computed by Open Epi- sample size calculation for cross sectional study to be 327 in each group, making a minimum total sample size of 654. However, in this study 678 pregnant women were recruited.
All eligible consented pregnant women with antenatal card (document showing number of visits and services provided) on admission were recruited into the study until a desired sample size was reached. Women without antenatal card at the time of admission were excluded from the study.
Ethical clearance was sought from the Department of Obstetrics and Gynecology and CUHAS/BMC Research Committee (CREC). A written informed consent was obtained from each participant after explaining the aims of the study and confidentiality was ensured throughout. Syphilis and HIV seropositive women were advised to attend at STI clinics with their partners for treatment, and were also linked to pediatricians for investigations and treatment of their babies. HIV positive women were linked to prevention of mother to child transmission (PMTCT) services for management.
Data, sample collection and laboratory diagnosis
A questionnaire was used to collect important parameters such as socio demographic data, gynecological and obstetric history and antenatal HIV status of study participants. The information given by patients was verified by crosschecking with antenatal cards information and results for syphilis tests and HIV tests were confirmed from the laboratory database and PMTCT unit. Venous blood (4mls) was taken using plain vacutainer tubes (BD, Nairobi, Kenya) and sent to the laboratory for rapid plasma reagin (RPR) test and reactive samples were confirmed by Immunochromatographic (rapid) treponemal test. Pre-test counseling and testing for HIV were done for pregnant women who had never had HIV testing and those screened negative at ANC visits following TANZANIA NATIONAL GUIDELINES ALGORITHMS.
The collected data were sorted, entered into Microsoft EXCEL software and then imported into STATA statistical package version 11 (Stata Corp LP College Station, Texas, USA) for consistent checks and cleaning. Analysis was done using the same package. Results were presented in percentages and odds ratio at 95% confidence level. Logistic regression analysis was done to test for the association.
Social demographic characteristics of study participants
Social demographic characteristics of study participants
Number = 663 (%)
Place of residence
Level of education
No formal education
Antenatal care profile of study participants
ANC services facility in index pregnancy, n = 663
Dispensary or health centre
GA(weeks) at first ANC visit, n = 663
ANC visits in index pregnancy, n = 663
GA(weeks) at ANC syphilis screening, n = 331
Reasons for not testing for syphilis at ANC, n = 332
Not informed to do a test
No personnel to do a test
Of 663 women enrolled, 331 (49.9%) were screened for syphilis during antenatal visit and 408 (61.5%) were screened for HIV during ANC. Of 663 women, 321 and 373 tested negative for syphilis and HIV during antenatal visits respectively. Of 321 who tested negative for syphilis at ANC 9 (2.7%) were seropositive at delivery while for 373 women who screened negative for HIV at ANC 8/373 (2%) were positive at delivery. Among those who did not test for syphilis and HIV at ANC 6 (1.8%) and 15/225 (6.6%) were positive for syphilis and HIV at delivery respectively.
Syphilis and HIV seroprevalence at delivery among study participants
ANC syphilis tests
Syphilis test results
HIV test results
Not tested, n = 332
6 (1. 8)
Tested negative, n = 321
Not tested (255)
Tested negative (373)
Factors associated with syphilis seropositivity at delivery: Logistic regression analysis
Positive n (%)
OR [95% CI]
OR [95% CI]
1.97 [0.255 - 15.198]
2.77 [0.602 - 12.807]
9.61 [3.28 - 28.35]
Not circumcised (192)
3.8 [1.4 – 10.85]
6.87 [2.313 - 20.437]
Number of partners
3.85 [0.503 -29.5]
Factors associated with HIV seropositivity at delivery: Logistic regression analysis
Positive n (%)
OR [95% CI]
OR [95% CI]
1.49 [0.255 – 3.300]
1.65 [0.600 - 4.894]
9.61 [3.27 - 28.305]
Not circumcised (192)
1.5 [0.667 – 2.330]
1.5 [0.806 - 2.893]
Number of Partners
1.37 [0.630 -3.018]
In this study, the prevalence of syphilis at delivery among pregnant women who did not test or tested negative for syphilis at ANC visits was 2.3%. This seroprevalence was lower than 9.3% reported in a study done in South Africa . The low prevalence of syphilis observed in this study could be explained by good health care seeking behaviour of the study population as it was observed that 60% of women in this study attended ANC at least four times which is above the national average of 42%. When compared to the previous studies in the same region the prevalence is low. The decrease in syphilis seroprevalence has been observed in other studies done in Mwanza region, Kenya, Botswana and Ethiopia [22–25]. The syphilis seroprevalence of 1.8% among women who did not test at ANC was also lower than the 8% and 18.2% previously reported in Mwanza and South Africa [19,21] respectively. Overall there was no significant difference between seroprevalence of HIV at delivery among those tested during antenatal and those tested during delivery. This has been observed previously  in Nigeria.
In this study syphilis seroconversion rate was 2.7% while that of HIV was 2%. The findings are comparable to the study done in South Africa  with the same rate for syphilis and even lower levels have been reported elsewhere . The HIV seroconversion rate observed in this study was inconsistent with a previous study  which reported seroconversion rate of 4.8%. This may be explained by well established HIV management and increased awareness among these women. The significance of retesting HIV late during the pregnancy is supported by previous studies [14,29,30] which indicated that some HIV positive babies are born to HIV seronegative mothers tested in early pregnancy.
In this study there were no association between syphilis seropositivity and maternal age during pregnancy (p = 0.515). Similar findings have been reported elsewhere in the country . In addition, maternal level of education in this study was not associated with syphilis seropositivity in pregnancy (p = 0.353) which is inconsistent with previous study done in Tanzania where syphilis seroprevalence was associated with low level of education among pregnant women . HIV seropositivity was significantly higher among syphilis seropositive pregnant women than among syphilis seronegative women (p < 0.001). Similar findings have been reported in other studies in northern Tanzania and in Kenya [33,34]. The syphilis and HIV co-infection rate of 0.9% observed in this study was lower than co-infection rates of 1.4% and 0.7% previously reported in other studies in Tanzania [24,32]. Male partner circumcision in this study was significantly associated with reduced syphilis seropositivity (p = 0.011), similar to what has been reported in study in Kenya . In our study, syphilis seropositivity was significantly associated with history of alcohol consumption (p < 0.001), similar findings have also been reported in previous studies [35,36]. Alcohol intake may be one of the many health related risks such women undertake, including concurrent multiple sexual partnership and high rate of unprotected sex that predispose to acquisition of Sexually transmitted infections (STIs) including syphilis. Our study confirmed the association between ulcerative STIs and HIV as it was observed that women with syphilis were nine times more at risk of acquiring HIV infection than those without syphilis.
One of the major limitation of this study was recruiting only pregnant women attending ANC. This might influence the seroprevalence of syphilis and HIV due to the fact that women with risk behaviours might not wish to attend ANC because of the routine screening of these infections.
Findings from this study suggest that women identified as negative for syphilis and HIV during ANC visits may seroconvert in the course of pregnancy and could benefit from re-screening. Delivery at health care facility also provides an opportunity for screening and treatment of women who missed out during the course of pregnancy. Seroconversion rates for syphilis and HIV observed in this study suggest that these women may potentially transmit these infections to the fetuses and affect the pregnancy outcome. This in turn justifies the need for re-screening for these infections especially in endemic areas in the third trimester and at delivery to reduce adverse perinatal outcomes associated with these infections.
Bugando medical centre
CUHAS/BMC Research Committee
Human immunodeficiency virus
Prevention of mother to child transmission
Rapid plasma regain
Sexually transmitted infections
World’s Health Organization
We are very grateful for the pregnant women who volunteered to participate in the study. We would like to acknowledge the assistance and guidance provided by all staff members of the Department of Obstetrics and gynecology and Care and treatment centre Bugando Medical Center, as well as the Department of Microbiology/Immunology, Catholic University of Health and Allied Sciences, Bugando Hospital. This research was supported by grants from Ministry of Health to JTDL and reagents from CUHAS to MMM.
- Labbe A-C, Mendonca AP, Alves AC, Jaffar S, Dias F, Alvarenga IC, et al. The impact of syphilis, HIV-1, and HIV-2 on pregnancy outcome in Bissau, Guinea-Bissau. Sex Transm Dis. 2002;29(3):157–67.View ArticlePubMedGoogle Scholar
- Organization WH: Sexually transmitted and other reproductive tract infections: a guide to essential practice. 2005 http://www.who.int/iris/handle/10665/43116.
- Organization WH: Special Programme for Research and Training in Tropical Diseases (WHO/TDR/SDI/06.1). The Use of Rapid Syphilis Tests. TDR/SDI; 2006 http://www.who.int/reproductivehealth/publications/rtis/TDR_SDI_06_1/en/.
- Genç M, Ledger WJ. Syphilis in pregnancy. Sex Transm Infect. 2000;76(2):73–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Organization WH: The global elimination of congenital syphilis: rationale and strategy for action 2007 http://www.who.int/reproductivehealth/publications/rtis/9789241595858/en/.
- Krüger C, Malleyeck I. Congenital syphilis: still a serious, under-diagnosed threat for children in resource-poor countries. World J Pediatr. 2010;6(2):125–31.View ArticlePubMedGoogle Scholar
- Organization WH: Global strategy for the prevention and control of sexually transmitted infections: 2006–2015: breaking the chain of transmission. 2007 http://www.who.int/hiv/pub/toolkits/stis_strategyen.pdf.
- Organization WH. Unicef. Global HIV/AIDS response: epidemic update and health sector progress towards universal access: progress report 2011. Geneva: World Health Organization; 2011.Google Scholar
- UNAIDS JUNPoHA. AIDS epidemic update: December 1999. AIDS Analysis Afr. 2000;10(5):2.Google Scholar
- Moodley D, Esterhuizen TM, Pather T, Chetty V, Ngaleka L. High HIV incidence during pregnancy: compelling reason for repeat HIV testing. Aids. 2009;23(10):1255–9.View ArticlePubMedGoogle Scholar
- Gray RH, Li X, Kigozi G, Serwadda D, Brahmbhatt H, Wabwire-Mangen F, et al. Increased risk of incident HIV during pregnancy in Rakai, Uganda: a prospective study. Lancet. 2005;366(9492):1182–8.View ArticlePubMedGoogle Scholar
- Sheffield JS, Wendel GD, McIntire DD, Norgard MV. The effect of progesterone levels and pregnancy on HIV-1 coreceptor expression. Reprod Sci. 2009;16(1):20–31.View ArticlePubMedGoogle Scholar
- McIntyre J. Mothers infected with HIV Reducing maternal death and disability during pregnancy. Br Med Bull. 2003;67(1):127–35.View ArticlePubMedGoogle Scholar
- Nielsen Saines K, Melo M, Varella I, Fonseca R, Lira R, Turella ML, et al. Primary HIV-1 infection during pregnancy: high rate of HIV-1 MTCT in a cohort of patients in southern brazil. Retrovirology. 2008;5(supplement 1):585–8.Google Scholar
- Guay LA, Musoke P, Fleming T, Bagenda D, Allen M, Nakabiito C, et al. Intrapartum and neonatal single-dose nevirapine compared with zidovudine for prevention of mother-to-child transmission of HIV-1 in Kampala, Uganda: HIVNET 012 randomised trial. Lancet. 1999;354(9181):795–802.View ArticlePubMedGoogle Scholar
- Statistics NB, Macro O. Tanzania Demographic and Health Survey 2004–05. Tanzania: National Bureau of Statistics and ORC Macro Dar es Salaam; 2005.Google Scholar
- Temmerman M: Integrating STI/RTI Care for Reproductive Health (Sexually Transmitted and Other Reproductive Tract Infections). WHO Guide to Esssential Practice 2005.
- Kelsey JL. Methods in Observational Epidemiology. Volume 26. Oxford: Oxford University Press; 1996.Google Scholar
- Watson-Jones D, Changalucha J, Gumodoka B, Weiss H, Rusizoka M, Ndeki L, et al. Syphilis in pregnancy in Tanzania. I. Impact of maternal syphilis on outcome of pregnancy. J Infect Dis. 2002;186(7):940–7.View ArticlePubMedGoogle Scholar
- Qolohle DC, Hoosen AA, Moodley J, Smith AN, Mlisana KP. Serological screening for sexually transmitted infections in pregnancy: is there any value in re-screening for HIV and syphilis at the time of delivery? Genitourin Med. 1995;71(2):65–7.PubMedPubMed CentralGoogle Scholar
- Qolohle D, Hoosen A, Moodley J, Smith A, Mlisana K. Serological screening for sexually transmitted infections in pregnancy: is there any value in re-screening for HIV and syphilis at the time of delivery? Genitourin Med. 1995;71(2):65–7.PubMedPubMed CentralGoogle Scholar
- Otieno-Nyunya B, Bennett E, Bunnell R, Dadabhai S, Gichangi A, Mugo N, et al. Epidemiology of syphilis in Kenya: results from a nationally representative serological survey. Sex Transm Infect. 2011;87(6):521–5.View ArticlePubMedGoogle Scholar
- Mulu A, Kassu A, Tessema B, Yismaw G, Tiruneh M, Moges F, et al. Seroprevalence of syphilis and HIV-1 during pregnancy in a teaching hospital in northwest Ethiopia. Jpn J Infect Dis. 2007;60(4):193.PubMedGoogle Scholar
- Kumogola Y, Slaymaker E, Zaba B, Mngara J, Isingo R, Changalucha J, et al. Trends in HIV & syphilis prevalence and correlates of HIV infection: results from cross-sectional surveys among women attending ante-natal clinics in Northern Tanzania. BMC Public Health. 2010;10(1):553.View ArticlePubMedPubMed CentralGoogle Scholar
- Creek T, Thuku H, Kolou B, Rahman M, Kilmarx P. Declining syphilis prevalence among pregnant women in northern Botswana: an encouraging sign for the HIV epidemic? Sex Transm Infect. 2005;81(6):453–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Okeudo C, Ezem B, Ojiyi E. Maternal HIV positive sero-prevalence at delivery at a tertiary hospital in South-Eastern Nigeria. Nigerian J Med. 2011;19(4):471–4.Google Scholar
- Lumbiganon P, Piaggio G, Villar J, Pinol A, Bakketeig L, Bergsjo P, et al. The epidemiology of syphilis in pregnancy. Int J STD AIDS. 2002;13(7):486–94.View ArticlePubMedGoogle Scholar
- Mbizvo M, Kasule J, Mahomed K, Nathoo K. HIV-1 seroconversion incidence following pregnancy and delivery among women seronegative at recruitment in Harare, Zimbabwe. Central Afr J Med. 2001;47(5):115–8.Google Scholar
- Lu L, Legwaila K, Motswere C, Smit M, Jimbo W, Creek T: HIV incidence in pregnancy and the first post-partum year and implications for PMTCT programs, Francistown, Botswana, 2008. In: 2009. Abstract; 2009 http://i-base.info/htb/1425
- Singh S, Lampe M, Babu AS, Rao S, Borkowf C, Nesheim S, et al. HIV seroconversion during pregnancy and mother-to-child HIV transmission: data from the enhanced perinatal surveillance project, United States, 2005–2010. J Int AIDS Soc 2012. 2012;1202:130–1.Google Scholar
- Yahya-Malima KI, Evjen-Olsen B, Matee MI, Fylkesnes K, Haarr L. HIV-1, HSV-2 and syphilis among pregnant women in a rural area of Tanzania: prevalence and risk factors. BMC Infect Dis. 2008;8(1):75.View ArticlePubMedPubMed CentralGoogle Scholar
- Swai RO, Matee MI, Killewo J, Lyamuya EF, Kwesigabo G, Tulli T, et al. Surveillance of HIV and syphilis infections among antenatal clinic attendees in Tanzania-2003/2004. BMC Public Health. 2006;6(1):91.View ArticlePubMedPubMed CentralGoogle Scholar
- Msuya SE, Uriyo J, Hussain A, Mbizvo EM, Jeansson S, Sam NE, Stray-Pedersen B: Prevalence of sexually transmitted infections among pregnant women with known HIV status in northern Tanzania. Reprod Health 2009, 6(4) doi:10.1186/1742-4755-6-4
- Temmerman M, Ali FM, Ndinya-Achola J, Moses S, Plummer FA, Piot P. Rapid increase of both HIV-1 infection and syphilis among pregnant women in Nairobi. Kenya Aids. 1992;6(10):1181–6.View ArticlePubMedGoogle Scholar
- Ghebremichael M, Paintsil E, Larsen U. Alcohol abuse, sexual risk behaviors and sexually transmitted infections in women in Moshi urban district, northern Tanzania. Sex Transm Dis. 2009;36(2):102.View ArticlePubMedPubMed CentralGoogle Scholar
- Cook RL, Clark DB. Is there an association between alcohol consumption and sexually transmitted diseases? A systematic review. Sex Transm Dis. 2005;32(3):156–64.View ArticlePubMedGoogle Scholar
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